Quantitative trait loci-dependent analysis of a gene co-expression network associated with Fusarium head blight resistance in bread wheat (Triticum aestivumL.)

BMC Genomics

Research Article

Christian Ametz¹  Hermann Buerstmayr¹  Wolfgang Schweiger¹  Gerald Siegwart¹  Barbara Steiner¹  Marc Lemmens¹  Manuel Spannagl²  Thomas Nussbaumer²  Klaus FX Mayer²  Karl G Kugler² 

[1] Institute for Biotechnology in Plant Production, IFA-Tulln, University of Natural Resources and Life Sciences, A-3430, Tulln, Austria;Munich Information Center for Protein Sequences/Institute for Bioinformatics and Systems Biology, Helmholtz Center Munich, D-85764, Neuherberg, Germany;

关键词: Triticum aestivum;    Bread wheat;    Fusarium graminearum;    Fusarium head blight;    Fhb1;    Qfhs.ifa-5A;    Transcriptome;    Gene co-expression network;    RNA-seq;   

DOI  :  10.1186/1471-2164-14-728

 received in 2013-06-25, accepted in 2013-10-14,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundFusarium head blight (FHB) caused by Fusarium graminearum Schwabe is one of the most prevalent diseases of wheat (Triticum aestivum L.) and other small grain cereals. Resistance against the fungus is quantitative and more than 100 quantitative trait loci (QTL) have been described. Two well-validated and highly reproducible QTL, Fhb1 and Qfhs.ifa-5A have been widely investigated, but to date the underlying genes have not been identified.ResultsWe have investigated a gene co-expression network activated in response to F. graminearum using RNA-seq data from near-isogenic lines, harboring either the resistant or the susceptible allele for Fhb1 and Qfhs.ifa-5A. The network identified pathogen-responsive modules, which were enriched for differentially expressed genes between genotypes or different time points after inoculation with the pathogen. Central gene analysis identified transcripts associated with either QTL within the network. Moreover, we present a detailed gene expression analysis of four gene families (glucanases, NBS-LRR, WRKY transcription factors and UDP-glycosyltransferases), which take prominent roles in the pathogen response.ConclusionsA combination of a network-driven approach and differential gene expression analysis identified genes and pathways associated with Fhb1 and Qfhs.ifa-5A. We find G-protein coupled receptor kinases and biosynthesis genes for jasmonate and ethylene earlier induced for Fhb1. Similarly, we find genes involved in the biosynthesis and metabolism of riboflavin more abundant for Qfhs.ifa-5A.

【 授权许可】

Unknown   
© Kugler et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.