Supplemental invasion of Salmonella from the perspective of Salmonella enterica serovars Kentucky and Typhimurium

BMC Microbiology

Research Article

Attila Karsi¹  Mark L. Lawrence¹  Robert Wills²  R. Hartford Bailey²  Sanaz Salehi²  Kevin Howe²  John P. Brooks³ 

[1] Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, MS, USA;Department of Pathobiology and Population Medicine, College of Veterinary Medicine, Mississippi State University, Mississippi State, Mississippi State, MS, USA;USDA-ARS, Genetics and Precision Agriculture Unit, Mississippi State, MS, USA;

关键词: Salmonella;    Kentucky;    Typhimurium;    Type III Secretion System;    Invasion;    Internalization;    Host Tropism;   

DOI  :  10.1186/s12866-017-0989-3

 received in 2016-08-12, accepted in 2017-03-23,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCritical to the development of Salmonellosis in humans is the interaction of the bacterium with the epithelial lining of the gastrointestinal tract. Traditional scientific reasoning held type III secretion system (T3SS) as the virulence factor responsible for bacterial invasion. In this study, field-isolated Salmonella enterica serovar Kentucky and a known human pathogen Salmonella enterica serovar Typhimurium were mutated and evaluated for the invasion of human colorectal adenocarcinoma epithelial cells.ResultsS. enterica serovar Kentucky was shown to actively invade a eukaryotic monolayer, though at a rate that was significantly lower than Typhimurium. Additionally, strains mutated for T3SS formation were less invasive than the wild-type strains, but the decrease in invasion was not significant in Kentucky.ConclusionsStrains mutated for T3SS formation were able to initiate invasion of the eukaryotic monolayer to varying degrees based on strain, In the case of Kentucky, the mutated strain initiated invasion at a level that was not significantly different from the wild-type strain. A different result was observed for Typhimurium as the mutation significantly lowered the rate of invasion in comparison to the wild-type strain.

【 授权许可】

CC BY   
© The Author(s). 2017