Sequence mining and transcript profiling to explore differentially expressed genes associated with lipid biosynthesis during soybean seed development

BMC Plant Biology

Research Article

Liang Liu¹  Xiu-Duo Fan¹  Guang Chen¹  Yuan-Yuan Dong²  Hai-Long Yin²  Ye-Peng Sun²  Xin-Yue Zhang²  Yu-Lin Li²  Nan Wang²  Fa-Wei Wang²  Yuan-Yuan Jing²  Xiao-Yan Li²  Huan Chen³  Hai-Yan Li³ 

[1] College of Life Sciences,Jilin Agricultural University, 130118, Changchun, Jilin, China;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, 130118, Changchun, Jilin, China;Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, 130118, Changchun, Jilin, China;College of Life Sciences,Jilin Agricultural University, 130118, Changchun, Jilin, China;

关键词: Gene expression;    Lipid;    RNA-sequencing;    Soybean;    Unigene;   

DOI  :  10.1186/1471-2229-12-122

 received in 2011-12-11, accepted in 2012-06-27,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundSoybean (Glycine max L.) is one of the most important oil crops in the world. It is desirable to increase oil yields from soybean, and so this has been a major goal of oilseed engineering. However, it is still uncertain how many genes and which genes are involved in lipid biosynthesis.ResultsHere, we evaluated changes in gene expression over the course of seed development using Illumina (formerly Solexa) RNA-sequencing. Tissues at 15 days after flowering (DAF) served as the control, and a total of 11592, 16594, and 16255 differentially expressed unigenes were identified at 35, 55, and 65 DAF, respectively. Gene Ontology analyses detected 113 co-expressed unigenes associated with lipid biosynthesis. Of these, 15 showed significant changes in expression levels (log2fold values ≥ 1) during seed development. Pathway analysis revealed 24 co-expressed transcripts involved in lipid biosynthesis and fatty acid biosynthesis pathways. We selected 12 differentially expressed genes and analyzed their expressions using qRT-PCR. The results were consistent with those obtained from Solexa sequencing.ConclusionThese results provide a comprehensive molecular biology background for research on soybean seed development, particularly with respect to the process of oil accumulation. All of the genes identified in our research have significance for breeding soybeans with increased oil contents.

【 授权许可】

CC BY   
© Chen et al.; licensee BioMed Central Ltd. 2012