Tumor and reproductive traits are linked by RNA metabolism genes in the mouse ovary: a transcriptome-phenotype association analysis

BMC Genomics

Proceedings

John J Sharp¹  Ulises Urzúa²  Gen-Mu Zhang³  Garrison A Owens⁴  David J Munroe⁴  James M Cherry⁴ 

[1] Center for Comparative Medicine, Baylor College of Medicine, One Baylor Plaza, 77030, Houston, TX, USA;Laboratorio de Genómica Aplicada, ICBM, Universidad de Chile, 1027, Independencia, Santiago, Chile;Laboratory Animal Sciences Program, SAIC-NCI Frederick, 21702, Frederick, MD, USA;Laboratory of Molecular Technology, SAIC-NCI Frederick, 915 Tollhouse Avenue, Suite 211, 21701, Frederick, MD, USA;

关键词: Vascular Endothelial Growth Factor;    Gene Ontology;    Granulosa Cell;    Litter Size;    Vascular Endothelial Growth Factor Level;   

DOI  :  10.1186/1471-2164-11-S5-S1

来源: Springer

【 摘 要 】

BackgroundThe link between reproductive life history and incidence of ovarian tumors is well known. Periods of reduced ovulations may confer protection against ovarian cancer. Using phenotypic data available for mouse, a possible association between the ovarian transcriptome, reproductive records and spontaneous ovarian tumor rates was investigated in four mouse inbred strains. NIA15k-DNA microarrays were employed to obtain expression profiles of BalbC, C57BL6, FVB and SWR adult ovaries.ResultsLinear regression analysis with multiple-test control (adjusted p ≤ 0.05) resulted in ovarian tumor frequency (OTF) and number of litters (NL) as the top-correlated among five tested phenotypes. Moreover, nearly one-hundred genes were coincident between these two traits and were decomposed in 76 OTF(–) NL(+) and 20 OTF(+) NL(–) genes, where the plus/minus signs indicate the direction of correlation. Enriched functional categories were RNA-binding/mRNA-processing and protein folding in the OTF(–) NL(+) and the OTF(+) NL(–) subsets, respectively. In contrast, no associations were detected between OTF and litter size (LS), the latter a measure of ovulation events in a single estrous cycle.ConclusionLiterature text-mining pointed to post-transcriptional control of ovarian processes including oocyte maturation, folliculogenesis and angiogenesis as possible causal relationships of observed tumor and reproductive phenotypes. We speculate that repetitive cycling instead of repetitive ovulations represent the actual link between ovarian tumorigenesis and reproductive records.

【 授权许可】

CC BY   
© Urzúa et al; licensee BioMed Central Ltd. 2010