Urine metabolic profile changes of CCl4-liver fibrosis in rats and intervention effects of Yi Guan Jian Decoction using metabonomic approach

BMC Complementary and Alternative Medicine

Research Article

Jianye Dai¹  Wenyu Wang¹  Yongyu Zhang¹  Xiaojun Gou²  Yu Zhao²  Jinghua Peng²  Qing Tao²  Qin Feng²  Ping Liu³  Yiyang Hu³ 

[1] Center for Traditional Chinese Medicine and Systems Biology of Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China;Institute of Liver Disease, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China;Institute of Liver Disease, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China;E-institute of Shanghai Municipal Education Commission, 201203, Shanghai, China;

关键词: Yi Guan Jian Decoction;    Carbon tetrachloride;    Liver fibrosis;    Urine metabolic profiles;    GC/MS;   

DOI  :  10.1186/1472-6882-13-123

 received in 2012-08-23, accepted in 2013-05-30,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundYi Guan Jian Decoction (YGJD), a famous Chinese prescription, has long been employed clinically to treat liver fibrosis. However, as of date, there is no report on the effects of YGJD from a metabonomic approach. In this study, a urine metabonomic method based on gas chromatography coupled with mass spectrometry (GC/MS) was employed to study the protective efficacy and metabolic profile changes caused by YGJD in carbon tetrachloride (CCl4)-induced liver fibrosis.MethodsUrine samples from Wistar rats of three randomly divided groups (control, model, and YGJD treated) were collected at various time-points, and the metabolic profile changes were analyzed by GC/MS with principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA). Furthermore, histopathology and biochemical examination were also carried out to ensure the success of CCl4-induced liver fibrosis model.ResultsUrine metabolic profile studies suggested distinct clustering of the three groups, and YGJD group was much closer to the control group by showing a tendency of recovering towards the control group. Fourteen significantly changed metabolites were found, and YGJD treatment could reverse the levels of these metabolites to normal levels or close to normal levels.ConclusionsThe current study indicates that the YGJD has significant anti-fibrotic effects on CCl4-induced liver fibrosis in rats, which might be by regulating the dysfunction of energy metabolism, amino acid metabolism, tryptophan metabolism, cytochrome P450 metabolism, and gut microflora metabolism. The metabonomic approach can be recommended to study the pharmacological effect and mechanism of complex Chinese medicines.

【 授权许可】

Unknown   
© Gou et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.