Different genetic architectures underlie crop responses to the same pathogen: the {Helianthus annuus * Phoma macdonaldii} interaction case for black stem disease and premature ripening

BMC Plant Biology

Research Article

Grégory Dechamp-Guillaume¹  Nicolas B. Langlade²  Stéphane Muños²  Nicolas Pouilly²  Patrick Vincourt²  Gwenaëlle Marchand³  Amandine Bordat⁴  Emmanuelle Bret-Mestries⁵ 

[1] ENSAT, UMR 1248 AGIR, BP52627, F-31326, Castanet-Tolosan, France;AGIR, Université de Toulouse, INRA, INPT, INP-EI PURPAN, Castanet-Tolosan, France;LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France;LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France;Present address: EURALIS Semences, Domaine de Sandreau, 6 Chemin de Panedautes, 31700, Mondonville, France;LIPM, Université de Toulouse, INRA, CNRS, Castanet-Tolosan, France;Present address: INRA, Université de Bordeaux, UMR 1332 de Biologie du Fruit et Pathologie, CS 20032, 33882, Villenave d’Ornon, France;Terres Inovia, UMR 1248 AGIR, BP52627, F-31326, Castanet-Tolosan, France;

关键词: Premature ripening;    Black stem;    Sunflower;    Phoma macdonaldii;    QTL mapping;   

DOI  :  10.1186/s12870-017-1116-1

 received in 2017-04-07, accepted in 2017-10-09,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundPhoma macdonaldii has been reported as the causal agent of black stem disease (BS) and premature ripening (PR) on sunflower. PR is considered as the most widespread and detrimental disease on sunflower in France. While genetic variability and QTL mapping for partial resistance of sunflower to stem, collar and roots attacks have been reported on plantlets in controlled conditions, this work aims to describe the genetic variability in a subset of a sunflower lines, and for the first time to map QTL involved in PR resistance evaluated in field conditions using controlled inoculation.ResultsAn efficient and reliable method for inoculation used in field experiments induced stem base necrosis on up to 98% of all plants. A significant genetic variability for PR resistance in the field was detected among the 20 inbred lines of the core collection tested across the two years. For QTL mapping, the PR resistance evaluation was performed on two recombinant inbred lines (RIL) populations derived from the crosses XRQxPSC8 and FUxPAZ2 in two different years. QTL analyses were based on a newly developed consensus genetic map comprising 1007 non-redundant molecular markers. In each of the two RIL populations, different QTL involved in PR partial sunflower resistance were detected. The most significant QTL were detected 49 days post infection (DPI) on LG10 (LOD 7.7) and on LG7 (LOD 12.1) in the XRQxPSC8 and FUxPAZ2 RIL population, respectively. In addition, different QTL were detected on both populations for PR resistance measured between 14 and 35 DPI. In parallel, the incidence of natural attack of P. macdonaldii resulting in BS disease was recorded, showing that in these populations, the genetic of resistance to both diseases is not governed by the same factors.ConclusionThis work provides the first insights on the genetic architecture of sunflower PR resistance in the field. Moreover, the separate studies of symptoms on different organs and in time series allowed the identification of a succession of genetic components involved in the sunflower resistance to PR and BS diseases caused by Phoma macdonaldii along the development of the {plant * pathogen} interaction.

【 授权许可】

CC BY   
© The Author(s). 2017