Biochar-amended potting medium reduces the susceptibility of rice to root-knot nematode infections

BMC Plant Biology

Research Article

Godelieve Gheysen¹  Tina Kyndt¹  Hong-li Ji²  Wen-kun Huang³  Jane Debode⁴ 

[1] Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium;Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium;Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Jingjusi Road 20, 610066, Chengdu, P. R. China;Department of Molecular Biotechnology, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 100193, Beijing, P. R. China;Plant Sciences Unit – Plant Protection, Institute for Agricultural and Fisheries Research (ILVO), Burg. van Gansberghelaan 96, 9820, Merelbeke, Belgium;

关键词: Biochar;    Meloidogyne graminicola;    Hydrogen peroxide;    Lignin;    Callose;   

DOI  :  10.1186/s12870-015-0654-7

 received in 2015-08-16, accepted in 2015-10-23,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundBiochar is a solid coproduct of biomass pyrolysis, and soil amended with biochar has been shown to enhance the productivity of various crops and induce systemic plant resistance to fungal pathogens. The aim of this study was to explore the ability of wood biochar to induce resistance to the root-knot nematode (RKN) Meloidogyne graminicola in rice (Oryza sativa cv. Nipponbare) and examine its histochemical and molecular impact on plant defense mechanisms.ResultsA 1.2 % concentration of biochar added to the potting medium of rice was found to be the most effective at reducing nematode development in rice roots, whereas direct toxic effects of biochar exudates on nematode viability, infectivity or development were not observed. The increased plant resistance was associated with biochar-primed H2O2 accumulation as well as with the transcriptional enhancement of genes involved in the ethylene (ET) signaling pathway. The increased susceptibility of the Ein2b-RNAi line, which is deficient in ET signaling, further confirmed that biochar-induced priming acts at least partly through ET signaling.ConclusionThese results suggest that biochar amendments protect rice plants challenged by nematodes. This priming effect partially depends on the ET signaling pathway and enhanced H2O2 accumulation.

【 授权许可】

CC BY   
© Huang et al. 2015