Broad-range potential of Asphodelus microcarpus leaves extract for drug development

BMC Microbiology

Research Article

Enzo Tramontano¹  Francesca Pintus¹  Aldo Frau¹  Antonella Fais¹  Amalia Di Petrillo¹  Vincenzo Piras²  Germano Orrù²  Antonello Mameli²  Ana M. González-Paramás³  Celestino Santos-Buelga³ 

[1] Department of Life and Environmental Sciences, University of Cagliari, Cittadella Universitaria, SS 554, Bivio per Sestu, I-09042, Cagliari, Monserrato, Italy;Department of Surgical Science, University of Cagliari, 09124, Cagliari, Italy;Grupo de Investigación en Polifenoles, Unidad de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Salamanca, Salamanca, Spain;

关键词: Asphodelus microcarpus;    Plant extract;    Antibiotics;    Biofilm;    Antimicrobial activity;    Antiviral response;    Ebola virus;   

DOI  :  10.1186/s12866-017-1068-5

 received in 2017-06-01, accepted in 2017-07-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMany plants have been used in traditional medicine for their antibacterial, antifungal, antiprotozoal, antiviral, antidiarrhoeal, analgesic, antimalarial, antioxidant, anti-inflammatory and anticancer activities.In order to find novel antimicrobial and antiviral agents, the aim of the present study was the evaluation of the antibacterial and antibiofilm susceptibility of Asphodelus microcarpus leaves extract. Moreover, the antiviral activity and the phytochemical composition of the active extract were also determined.MethodsAntimicrobial and antibiofilm activities of leaves ethanol extract of A. microcarpus were evaluated on 13 different microbial strains. We selected three different sets of microorganisms: (i) Gram-positive bacteria, (ii) Gram-negative bacteria and (iii) yeasts. The potential antiviral activity of A. microcarpus leaves ethanol extract was evaluated with a luciferase reporter gene assay in which the dsRNA-dependent RIG-I-mediated IFN-β activation was inducted or inhibited by the Ebola virus VP35 protein. HPLC-DAD-MS was used to identify phenolic profile of the active extract.ResultsA. microcarpus leaves extract showed a potent inhibitory activity on Gram-positive bacteria while only a reduced inhibition was observed on Gram-negative bacteria. No activity was detected against Yeasts. The extract also showed an interesting antibiofilm motif on various bacterial strains (E. coli, S. aureus, S. haemolyticus and B. clausii). Moreover, this extract significantly affected the Ebola virus VP35 inhibition of the viral RNA (vRNA) induced IFN response.ConclusionsThe overall results provide supportive data on the use of A. microcarpus as antimicrobial agent and a potential source of anti-viral natural products.Data collected set the bases for further studies for the identification of single active components and the development of new pharmaceuticals.

【 授权许可】

CC BY   
© The Author(s). 2017