An experimental study of VEGF induced changes in vasoactivity in pig retinal arterioles and the influence of an anti-VEGF agent

BMC Ophthalmology

Research Article

Ian L McAllister¹  Dao-Yi Yu²  Stephen J Cringle²  Er-Ning Su² 

[1] Centre for Ophthalmology and Visual Science, The University of Western Australia, Perth, Australia;Centre for Ophthalmology and Visual Science, The University of Western Australia, Perth, Australia;ARC Centre of Excellence in Vision Science, The University of Western Australia, Perth, Australia;

关键词: Vascular Endothelial Growth Factor;    Bevacizumab;    Retinal Vein Occlusion;    Central Retinal Vein Occlusion;    Branch Retinal Vein Occlusion;   

DOI  :  10.1186/1471-2415-12-10

 received in 2011-12-08, accepted in 2012-05-29,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundVascular endothelial growth factor (VEGF) plays an important role in ocular physiology. Anti-VEGF agents are now used for treatment of common retinal diseases. This study characterises the vasoactive properties of VEGF in isolated perfused pig retinal arterioles under normal tone or endothelin-1 (ET-1) pre-contracted conditions and determines the influence of an anti VEGF agent on VEGF induced vasoactivity.MethodsAn isolated perfused retinal arteriole preparation was used. The outer diameter of retinal vessels was monitored at 2 second intervals in response to VEGF and the anti VEGF agent, bevacizumab. The effect of intraluminal delivery of VEGF was determined over a wide concentration range (10-16 to 10-7 M) both with and without pre-contraction with ET-1 (3 x 10-9 M). Bevacizumab (0.35 mg mL-1) was applied extraluminally to determine the influence of bevacizumab on VEGF induced vasoactive changes on ET-1 pre-contracted vessels.ResultsIn retinal arterioles with normal tone, VEGF induced a concentration dependent contraction at low concentrations, reaching 93.5% at 10-11 M and then contraction was reduced at higher concentrations, recovering to 98.1% at 10-7 M. VEGF produced a potent concentration dependent vasodilatation in arterioles pre-contracted with ET-1. VEGF induced vasodilatation in arterioles pre-contracted with ET-1 was significantly inhibited by bevacizumab.ConclusionsVEGF induced vasoactive changes in pig retinal arterioles are dependent on concentration and vascular tone. Bevacizumab inhibits VEGF-induced vasodilatation in pre-contracted arterioles.

【 授权许可】

Unknown   
© Su et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.