The genome-wide transcription response to telomerase deficiency in the thermotolerant yeast Hansenula polymorpha DL-1

BMC Genomics

Research Article

Olga A. Petrova¹  Yulia Yu. Parfenova²  Maria P. Rubtsova²  Maria I. Zvereva²  Olga A. Dontsova³  Alexander N. Malyavko⁴  Andrey V. Mardanov⁵  Nikolai V. Ravin⁵  Alexey V. Beletsky⁵  Eugenia S. Mardanova⁵  Maria V. Sukhanova⁶  Olga I. Lavrik⁷ 

[1] Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory 1, bld. 40, 119992, Moscow, Russia;Faculty of Chemistry, Moscow State University, Leninskie Gory 1, bld. 3, 119991, Moscow, Russia;Faculty of Chemistry, Moscow State University, Leninskie Gory 1, bld. 3, 119991, Moscow, Russia;Belozersky Institute of Physico-Chemical Biology, Moscow State University, Leninskie Gory 1, bld. 40, 119992, Moscow, Russia;Center of Functional Genomics, Skolkovo Institute of Science and Technology, 143026, Moscow, Russia;Faculty of Chemistry, Moscow State University, Leninskie Gory 1, bld. 3, 119991, Moscow, Russia;Center of Functional Genomics, Skolkovo Institute of Science and Technology, 143026, Moscow, Russia;Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, bld 2, 119071, Moscow, Russia;Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentiev Ave. 8, 630090, Novosibirsk, Russia;Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, Lavrentiev Ave. 8, 630090, Novosibirsk, Russia;Novosibirsk State University, 630090, Novosibirsk, Russia;

关键词: Telomerase;    Senescence;    Yeast;    Hansenula polymorpha;    RNA-seq;    DNA repair;    Autophagy;    Environmental stress response;   

DOI  :  10.1186/s12864-017-3889-x

 received in 2017-04-22, accepted in 2017-06-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIn the course of replication of eukaryotic chromosomes, the telomere length is maintained due to activity of telomerase, the ribonucleoprotein reverse transcriptase. Abolishing telomerase function causes progressive shortening of telomeres and, ultimately, cell cycle arrest and replicative senescence. To better understand the cellular response to telomerase deficiency, we performed a transcriptomic study for the thermotolerant methylotrophic yeast Hansenula polymorpha DL-1 lacking telomerase activity.ResultsMutant strain of H. polymorpha carrying a disrupted telomerase RNA gene was produced, grown to senescence and analyzed by RNA-seq along with wild type strain. Telomere shortening induced a transcriptional response involving genes relevant to telomere structure and maintenance, DNA damage response, information processing, and some metabolic pathways. Genes involved in DNA replication and repair, response to environmental stresses and intracellular traffic were up-regulated in senescent H. polymorpha cells, while strong down-regulation was observed for genes involved in transcription and translation, as well as core histones.ConclusionsComparison of the telomerase deletion transcription responses by Saccharomyces cerevisiae and H. polymorpha demonstrates that senescence makes different impact on the main metabolic pathways of these yeast species but induces similar changes in processes related to nucleic acids metabolism and protein synthesis. Up-regulation of a subunit of the TORC1 complex is clearly relevant for both types of yeast.

【 授权许可】

CC BY   
© The Author(s). 2017