Global transcriptome response in Lactobacillus sakei during growth on ribose

BMC Microbiology

Research Article

Lars Snipen¹  Lars Axelsson²  Kristine Naterstad²  Anette McLeod³ 

[1] Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, 1432, Ås, NO, Norway;Nofima Mat AS,, orwegian Institute of Food, Fisheries and Aquaculture Research, Osloveien 1, 1430, Ås, NO, Norway;Nofima Mat AS,, orwegian Institute of Food, Fisheries and Aquaculture Research, Osloveien 1, 1430, Ås, NO, Norway;Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, P.O. Box 5003, 1432, Ås, NO, Norway;

关键词: Ribose;    Carbon Catabolite Repression;    Purine Nucleoside Phosphorylase;    Lactobacillus Sakei;    Nucleoside Hydrolase;   

DOI  :  10.1186/1471-2180-11-145

 received in 2011-02-09, accepted in 2011-06-24,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundLactobacillus sakei is valuable in the fermentation of meat products and exhibits properties that allow for better preservation of meat and fish. On these substrates, glucose and ribose are the main carbon sources available for growth. We used a whole-genome microarray based on the genome sequence of L. sakei strain 23K to investigate the global transcriptome response of three L. sakei strains when grown on ribose compared with glucose.ResultsThe function of the common regulated genes was mostly related to carbohydrate metabolism and transport. Decreased transcription of genes encoding enzymes involved in glucose metabolism and the L-lactate dehydrogenase was observed, but most of the genes showing differential expression were up-regulated. Especially transcription of genes directly involved in ribose catabolism, the phosphoketolase pathway, and in alternative fates of pyruvate increased. Interestingly, the methylglyoxal synthase gene, which encodes an enzyme unique for L. sakei among lactobacilli, was up-regulated. Ribose catabolism seems closely linked with catabolism of nucleosides. The deoxyribonucleoside synthesis operon transcriptional regulator gene was strongly up-regulated, as well as two gene clusters involved in nucleoside catabolism. One of the clusters included a ribokinase gene. Moreover, hprK encoding the HPr kinase/phosphatase, which plays a major role in the regulation of carbon metabolism and sugar transport, was up-regulated, as were genes encoding the general PTS enzyme I and the mannose-specific enzyme II complex (EIIman). Putative catabolite-responsive element (cre) sites were found in proximity to the promoter of several genes and operons affected by the change of carbon source. This could indicate regulation by a catabolite control protein A (CcpA)-mediated carbon catabolite repression (CCR) mechanism, possibly with the EIIman being indirectly involved.ConclusionsOur data shows that the ribose uptake and catabolic machinery in L. sakei is highly regulated at the transcription level. A global regulation mechanism seems to permit a fine tuning of the expression of enzymes that control efficient exploitation of available carbon sources.

【 授权许可】

CC BY   
© McLeod et al; licensee BioMed Central Ltd. 2011