Transcriptomic analysis of wound xylem formation in Pinus canariensis

BMC Plant Biology

Research Article

V. Chano¹  A. Soto²  C. Collada² 

[1] G.I. Genética, Fisiología e Historia Forestal. ETSI Montes, Forestal y del Medio Natural. Dpto. Sistemas y Recursos Naturales, Universidad Politécnica de Madrid, Ciudad Universitaria s, /n 28040, Madrid, Spain;G.I. Genética, Fisiología e Historia Forestal. ETSI Montes, Forestal y del Medio Natural. Dpto. Sistemas y Recursos Naturales, Universidad Politécnica de Madrid, Ciudad Universitaria s, /n 28040, Madrid, Spain;Unidad Mixta de Genómica y Ecofisiología Forestal INIA/UPM, Madrid, Spain;

关键词: Wound;    Healing;    Conifers;    Transcriptome;    Wood;    Pinus canariensis;   

DOI  :  10.1186/s12870-017-1183-3

 received in 2017-08-02, accepted in 2017-11-22,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWoody plants, especially trees, usually must face several injuries caused by different agents during their lives. Healing of injuries in stem and branches, affecting the vascular cambium and xylem can take several years. In conifers, healing takes place mainly from the remaining vascular cambium in the margin of the wound. The woundwood formed in conifers during healing usually presents malformed and disordered tracheids as well as abundant traumatic resin ducts. These characteristics affect its functionality as water conductor and its technological properties.ResultsIn this work we analyze for the first time the transcriptomic basis of the formation of traumatic wood in conifers, and reveal some differences with normal early- and late-wood. Microarray analysis of the differentiating traumatic wood, confirmed by quantitative RT-PCR, has revealed alterations in the transcription profile of up to 1408 genes during the first period of healing. We have grouped these genes in twelve clusters, according to their transcription profiles, and have distinguished accordingly two main phases during this first healing.ConclusionsWounding induces a complete rearrangement of the transcriptional program in the cambial zone close to the injuries. At the first instance, radial growth is stopped, and a complete set of defensive genes, mostly related to biotic stress, are induced. Later on, cambial activity is restored in the lateral borders of the wound, even at a high rate. During this second stage certain genes related to early-wood formation, including genes involved in cell wall formation and transcription factors, are significantly overexpressed, while certain late-wood related genes are repressed. Additionally, significant alterations in the transcription profile of abundant non annotated genes are reported.

【 授权许可】

CC BY   
© The Author(s). 2017