Transcriptome profiling of a spirodiclofen susceptible and resistant strain of the European red mite Panonychus ulmi using strand-specific RNA-seq

BMC Genomics

Research Article

Ralf Nauen¹  Robert Greenhalgh²  Richard M. Clark³  Sabina Bajda⁴  Thomas Van Leeuwen⁵  Wannes Dermauw⁶  Luc Tirry⁶ 

[1] Bayer CropScience AG, Research Pest Control, Alfred Nobel Str. 50, D-40789, Monheim, Germany;Department of Biology, University of Utah, 257 South 1400 East, 84112, Salt Lake City, UT, USA;Department of Biology, University of Utah, 257 South 1400 East, 84112, Salt Lake City, UT, USA;Center for Cell and Genome Science, University of Utah, 257 South 1400 East, 84112, Salt Lake City, UT, USA;Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, P.O. Box 9424, 1090, Amsterdam, GE, The Netherlands;Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, P.O. Box 9424, 1090, Amsterdam, GE, The Netherlands;Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium;Laboratory of Agrozoology, Department of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, B-9000, Ghent, Belgium;

关键词: Cyclic keto-enols;    Tetranychidae;    Target-site mutation;    biRNA virus;    Spirotetramat;    Intradiol ring-cleavage dioxygenases;    UDP-glycosyltransferases;    Cytochrome P450 mono-oxygenases;    Carboxyl/cholinesterases;    Gluthathione-S-transferases;   

DOI  :  10.1186/s12864-015-2157-1

 received in 2015-07-13, accepted in 2015-10-27,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe European red mite, Panonychus ulmi, is among the most important mite pests in fruit orchards, where it is controlled primarily by acaricide application. However, the species rapidly develops pesticide resistance, and the elucidation of resistance mechanisms for P. ulmi has not kept pace with insects or with the closely related spider mite Tetranychus urticae. The main reason for this lack of knowledge has been the absence of genomic resources needed to investigate the molecular biology of resistance mechanisms.ResultsHere, we provide a comprehensive strand-specific RNA-seq based transcriptome resource for P. ulmi derived from strains susceptible and resistant to the widely used acaricide spirodiclofen. From a de novo assembly of the P. ulmi transcriptome, we manually annotated detoxification enzyme families, target-sites of commonly used acaricides, and horizontally transferred genes implicated in plant-mite interactions and pesticide resistance. In a comparative analysis that incorporated sequences available for Panonychus citri, T. urticae, and insects, we identified radiations for detoxification gene families following the divergence of Panonychus and Tetranychus genera. Finally, we used the replicated RNA-seq data from the spirodiclofen susceptible and resistant strains to describe gene expression changes associated with resistance. A cytochrome P450 monooxygenase, as well as multiple carboxylcholinesterases, were differentially expressed between the susceptible and resistant strains, and provide a molecular entry point for understanding resistance to spirodiclofen, widely used to control P. ulmi populations.ConclusionsThe new genomic resources and data that we present in this study for P. ulmi will substantially facilitate molecular studies of underlying mechanisms involved in acaricide resistance.

【 授权许可】

CC BY   
© Bajda et al. 2015