Global analysis of protein lysine succinylation profiles in common wheat

BMC Genomics

Research Article

Qi Lin¹  Yumei Zhang¹  Limin Song¹  Ping Mu¹  Wenxing Liang¹  Shu Wang²  Guangyuan Wang³ 

[1] College of Agronomy and Plant Protection, Qingdao Agricultural University, 266109, Qingdao, Shandong, China;College of Agronomy, Shenyang Agricultural University, 110866, Shenyang, Liaoning, China;College of Life Sciences, Shandong Province Key Laboratory of Applied Mycology, Qingdao Agricultural University, 266109, Qingdao, China;

关键词: Lysine succinylation;    Succinylome;    Triticum aestivum;    Post-translational modification;    Photosynthesis;   

DOI  :  10.1186/s12864-017-3698-2

 received in 2016-09-30, accepted in 2017-04-11,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundProtein lysine succinylation is an important post-translational modification and plays a critical regulatory role in almost every aspects of cell metabolism in both eukaryotes and prokaryotes. Common wheat is one of the major global cereal crops. However, to date, little is known about the functions of lysine succinylation in this plant. Here, we performed a global analysis of lysine succinylation in wheat and examined its overlap with lysine acetylation.ResultsIn total, 330 lysine succinylated modification sites were identified in 173 proteins. Bioinformatics analysis showed that the modified proteins are distributed in multiple subcellular compartments and are involved in a wide variety of biological processes such as photosynthesis and the Calvin-Benson cycle, suggesting an important role for lysine succinylation in these processes. Five putative succinylation motifs were identified. A protein interaction network analysis revealed that diverse interactions are modulated by protein succinylation. Moreover, 21 succinyl-lysine sites were found to be acetylated at the same position, and 33 proteins were modified by both acetylation and succinylation, suggesting an extensive overlap between succinylation and acetylation in common wheat. Comparative analysis indicated that lysine succinylation is conserved between common wheat and Brachypodium distachyon.ConclusionsThese results suggest that lysine succinylation is involved in diverse biological processes, especially in photosynthesis and carbon fixation. This systematic analysis represents the first global analysis of lysine succinylation in common wheat and provides an important resource for exploring the physiological role of lysine succinylation in this cereal crop and likely in all plants.

【 授权许可】

CC BY   
© The Author(s). 2017