Millicurrent stimulation of human articular chondrocytes cultivated in a collagen type-I gel and of human osteochondral explants

BMC Complementary and Alternative Medicine

Research Article

Karsten Gavénis¹  Stefan Andereya¹  Ralf Mueller-Rath¹  Bernhard Schmidt-Rohlfing²  Jiri Silny³  Ulrich Schneider⁴ 

[1] Aachen University Hospital, Dept of Orthopaedic Surgery, Pauwelsstr. 30, 52074, Aachen, Germany;Aachen University Hospital, Dept of Trauma Surgery, Pauwelsstr. 30, 52074, Aachen, Germany;Aachen University Hospital, Research Center for Bioelectromagnetic Interaction (femu), Pauwelsstr. 30, 52074, Aachen, Germany;Arthro Nova Clinic, Wiesseerstr. 103, 83707, Ringsee, Germany;

关键词: Total Knee Replacement;    Articular Chondrocytes;    Hyaline Cartilage;    Human Chondrocytes;    Cartilage Tissue Engineering;   

DOI  :  10.1186/1472-6882-10-43

 received in 2010-02-23, accepted in 2010-08-06,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundHere we investigate the effect of millicurrent treatment on human chondrocytes cultivated in a collagen gel matrix and on human osteochondral explants.MethodsHuman chondrocytes from osteoarthritic knee joints were enzymatically released and transferred into a collagen type-I gel. Osteochondral explants and cell-seeded gel samples were cultivated in-vitro for three weeks. Samples of the verum groups were stimulated every two days by millicurrent treatment (3 mA, sinusoidal signal of 312 Hz amplitude modulated by two super-imposed signals of 0.28 Hz), while control samples remained unaffected. After recovery, collagen type-I, type-II, aggrecan, interleukin-1β, IL-6, TNFα and MMP13 were examined by immunohistochemistry and by real time PCR.ResultsWith regard to the immunostainings 3 D gel samples and osteochondral explants did not show any differences between treatment and control group. The expression of all investigated genes of the 3 D gel samples was elevated following millicurrent treatment. While osteochondral explant gene expression of col-I, col-II and Il-1β was nearly unaffected, aggrecan gene expression was elevated. Following millicurrent treatment, IL-6, TNFα, and MMP13 gene expression decreased. In general, the standard deviations of the gene expression data were high, resulting in rarely significant results.ConclusionsWe conclude that millicurrent stimulation of human osteoarthritic chondrocytes cultivated in a 3 D collagen gel and of osteochondral explants directly influences cell metabolism.

【 授权许可】

CC BY   
© Gavénis et al; licensee BioMed Central Ltd. 2010