Development of transgenic wheat (Triticum aestivum L.) expressing avidin gene conferring resistance to stored product insects

BMC Plant Biology

Research Artic

Hanan A Nour-Eldin¹  Sameh E Hassanein¹  Adel A Gad¹  Yasser B Morsy¹  Reda Salem¹  Mohamed T Abdelsattar¹  Hala F Eissa²  Ahmed M Ramadan³  Gamal H Osman⁴  Doaa K El-Ghareeb⁴  Hussien F Alameldin⁵  Ahmed Bahieldin⁶  Maher M Shehata⁷  Hala M Mahfouz⁷  Soheir E Elkhodary⁷  Heba H Abouseadaa⁷ 

[1] Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), 12619, Giza, Egypt;Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), 12619, Giza, Egypt;College of Biotechnology, Misr University of Science and technology, Giza, Egypt;Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), 12619, Giza, Egypt;Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), 80203, Jeddah, Saudi Arabia;Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), 12619, Giza, Egypt;Department of Biology, Faculty of Applied Sciences, Umm Al Qura University, 21955, Makkah, Saudi Arabia;Agricultural Genetic Engineering Research Institute (AGERI), Agriculture Research Center (ARC), 12619, Giza, Egypt;Plant Soil and Microbial Sciences Department, Michigan state University, M9, 48824, East Lansing, USA;Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), 80203, Jeddah, Saudi Arabia;Department of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, Egypt;Department of Botany, Faculty of Science, Ain Shams University, Cairo, Egypt;

关键词: Stored cereals insects;    post harvest loss;    avidin;    transgenic wheat;   

DOI  :  10.1186/s12870-015-0570-x

 received in 2015-05-10, accepted in 2015-07-07,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundWheat is considered the most important cereal crop all over the world. The wheat weevil Sitophilus granarius is a serious insect pests in much of the wheat growing area worldwide and is responsible for significant loss of yield. Avidin proteins has been proposed to function as plant defense agents against insect pests.ResultsA synthetic avidin gene was introduced into spring wheat (Triticum aestivum L.) cv. Giza 168 using a biolistic bombardment protocol. The presence and expression of the transgene in six selected T0 transgenic wheat lines were confirmed at the molecular level. Accumulation of avidin protein was detected in transgenic plants compared to non-transgenic plants. Avidin transgene was stably integrated, transcribed and translated as indicated by Southern blot, ELISA, and dot blot analyses, with a high level of expression in transgenic wheat seeds. However, no expression was detected in untransformed wheat seeds. Functional integrity of avidin was confirmed by insect bioassay. The results of bioassay using transgenic wheat plants challenged with wheat weevil revealed 100 % mortality of the insects reared on transgenic plants after 21 days.ConclusionTransgenic wheat plants had improved resistance to Sitophilus granarius.

【 授权许可】

Unknown   
© Abouseadaa et al. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.