A 90 day chronic toxicity study of Nigerian herbal preparation DAS-77 in rats

BMC Complementary and Alternative Medicine

Research Article

Chidozie C Anunobi¹  Olufunmilayo O Adeyemi²  Saheed O Afolabi²  Olufunsho Awodele²  Abidemi J Akindele² 

[1] Department of Morbid Anatomy, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P. M. B, 12003, Lagos, Nigeria;Department of Pharmacology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P. M. B, 12003, Lagos, Nigeria;

关键词: DAS-77;    Herbal preparation;    Toxicological assessment;    Acute toxicity;    Chronic toxicity;   

DOI  :  10.1186/1472-6882-12-79

 received in 2012-01-17, accepted in 2012-06-15,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundThe herbal preparation DAS-77, used for the treatment of various ailments in Nigeria, contains the milled bark of Mangifera indica L. and root of Carica papaya L. Toxicological assessment of the preparation was carried out in this study.MethodsIn the acute toxicity study, DAS-77 was administered to mice p.o. up to 20 g/kg in divided doses and i.p. at 250–3000 mg/kg. Mortality within 24 h was recorded. In the chronic toxicity study, rats were treated p.o. for 90 days at doses of 80, 400 (therapeutic dose, TD) and 2000 mg/kg. By 90 days, animals were sacrificed and blood samples collected for hematological and biochemical analysis. Organs were harvested for weight determination, antioxidants and histopathological assessments.ResultsDAS-77 did not produce any lethality administered p.o. up to 20 g/kg in divided doses but the i.p. LD50 was 1122.0 mg/kg. At TD, DAS-77 produced significant (p < 0.05) reductions in body weight, food intake and K+, and increases in ovary weight, neutrophils and HDL, which were reversible. Histopathological presentations were generally normal. Effects at the other doses were comparable to those at TD except for reversible increases in antioxidants in the liver, kidney and testes, and sperm abnormality, and reductions in liver enzymes, sperm motility and count.ConclusionsFindings in this study revealed that DAS-77 is relatively safe with the potential for enhancing in vivo antioxidant activity. However, possibly reversible side-effects include electrolyte imbalance and sterility in males.

【 授权许可】

Unknown   
© Afolabi et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.