Diversity and biofilm-production ability among isolates of Escherichia coli phylogroup D belonging to ST69, ST393 and ST405 clonal groups

BMC Microbiology

Research Article

Carolina Montenegro¹  Luísa Peixe¹  João Pires¹  Ângela Novais¹  Gianfranco Donelli²  Claudia Vuotto²  Teresa M Coque³ 

[1] Departamento de Microbiologia, REQUIMTE, Faculdade Farmácia Universidade Porto, Rua Jorge Viterbo Ferreira n° 228, 4050–313, Porto, Porto, Portugal;Microbial Biofilm Laboratory (LABIM), IRCCS Fondazione Santa Lucia, Rome, Italy;Servicio de Microbiología and CIBER en Epidemiología y Salud Pública (CIBERESP), Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Hospital Universitario Ramón y Cajal, Madrid, Spain;

关键词: ExPEC;    High-risk clones;    ESBL;    Virulence;    Adhesion;    Biofilm;   

DOI  :  10.1186/1471-2180-13-144

 received in 2013-03-05, accepted in 2013-06-17,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundPhylogenetic group D Escherichia coli clones (ST69, ST393, ST405) are increasingly reported as multidrug resistant strains causing extra-intestinal infections. We aim to characterize inter- and intraclonal diversity of a broad sample (isolates from different geographic locations and origins with variable antibiotic resistance profiles, 1980-2010) and their ability to adhere and form biofilm by both a modified quantitative biofilm producing assay and Field Emission Scanning Electron Microscopy (FESEM).ResultsHigh virulence scores were observed among ST69 (median 14/range 9–15) and ST393 (median 14/range 8–15) clones, particularly enriched in pap alleles, iha, kpsMTII-K5 and ompT, in contrast with ST405 (median 6/range 2–14) isolates, exhibiting frequently fyuA, malX and traT. All ST69 and ST393 and only two ST405 isolates were classified as ExPEC. Biofilm production was detected in two non-clinical ST69 and three ST393 isolates from different origins showing variable virulence profiles. Within each clonal group, and despite the high diversity of PFGE-types observed, isolates from different countries and recovered over large periods of time were clustered in a few groups sharing common virulence gene profiles among ST69 (n = 10 isolates) and ST393 (n = 9 isolates) (fimH-iha-iutA-kpsMTII-K5-(traT)-sat-(ompT)-papA-papEF-papGII-papC) or ST405 (n = 6 isolates) (fimH-traT-fyuA-malX).ConclusionsThis study highlights the circulation of highly transmissible ST69, ST393 and ST405 variants among different settings. Biofilm production seems not to be directly correlated with their epidemiological success.

【 授权许可】

CC BY   
© Novais et al.; licensee BioMed Central Ltd. 2013