Functional blockade of α5β1 integrin induces scattering and genomic landscape remodeling of hepatic progenitor cells

BMC Cell Biology

Research Article

Luciano Vellón¹  Félix Royo¹  Luis A Parada²  Rune Matthiesen³  José Torres-Fuenzalida⁴  Alicia Lorenti⁵ 

[1] Cytogenomics, CIC bioGUNE-CIBEREHD, Par. Tec. Bizkaia Ed. 801 A, 48160, Derio, Spain;Cytogenomics, CIC bioGUNE-CIBEREHD, Par. Tec. Bizkaia Ed. 801 A, 48160, Derio, Spain;Institute of Experimental Pathology, Faculty of Health Sciences, National University of Salta, Bolivia 5010, 4400, Salta, Argentina;IPATIMUP - Institute of Molecular Pathology and Immunology, University of Porto, Portugal;Institute for Basic Sciences and Experimental Medicine, Hospital Italiano, Potosi 4240, 1199, Buenos Aires, Argentina;Unidad de Traducción Clínica - Ingeniería de Tejidos, Austral University, Pte Perón 1500, 1635, Pilar, Argentina;

关键词: Integrin;    Wound Healing Assay;    Transwell Assay;    Cell Scattering;    Hepatic Progenitor Cell;   

DOI  :  10.1186/1471-2121-11-81

 received in 2010-04-16, accepted in 2010-10-19,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundCell scattering is a physiological process executed by stem and progenitor cells during embryonic liver development and postnatal organ regeneration. Here, we investigated the genomic events occurring during this process induced by functional blockade of α5β1 integrin in liver progenitor cells.ResultsCells treated with a specific antibody against α5β1 integrin exhibited cell spreading and scattering, over-expression of liver stem/progenitor cell markers and activation of the ERK1/2 and p38 MAPKs signaling cascades, in a similar manner to the process triggered by HGF/SF1 stimulation. Gene expression profiling revealed marked transcriptional changes of genes involved in cell adhesion and migration, as well as genes encoding chromatin remodeling factors. These responses were accompanied by conspicuous spatial reorganization of centromeres, while integrin genes conserved their spatial positioning in the interphase nucleus.ConclusionCollectively, our results demonstrate that α5β1 integrin functional blockade induces cell migration of hepatic progenitor cells, and that this involves a dramatic remodeling of the nuclear landscape.

【 授权许可】

Unknown   
© Vellón et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.