Genetic analysis, structural modeling, and direct coupling analysis suggest a mechanism for phosphate signaling in Escherichia coli

BMC Genetics

Research

Justin B Miller¹  Perry G Ridge¹  Tanner Dean²  Stewart G Gardner²  Tanner Robinson²  McCall Erickson²  William R McCleary² 

[1] Department of Biology, Brigham Young University, Provo, UT, USA;Microbiology and Molecular Biology Department, Brigham Young University, Provo, UT, USA;

关键词: PhoR;    PhoU;    PstSCAB;    Pho regulon;    two component signal transduction;    PAS domain;    direct coupling analysis;   

DOI  :  10.1186/1471-2156-16-S2-S2

来源: Springer

【 摘 要 】

BackgroundProper phosphate signaling is essential for robust growth of Escherichia coli and many other bacteria. The phosphate signal is mediated by a classic two component signal system composed of PhoR and PhoB. The PhoR histidine kinase is responsible for phosphorylating/dephosphorylating the response regulator, PhoB, which controls the expression of genes that aid growth in low phosphate conditions. The mechanism by which PhoR receives a signal of environmental phosphate levels has remained elusive. A transporter complex composed of the PstS, PstC, PstA, and PstB proteins as well as a negative regulator, PhoU, have been implicated in signaling environmental phosphate to PhoR.ResultsThis work confirms that PhoU and the PstSCAB complex are necessary for proper signaling of high environmental phosphate. Also, we identify residues important in PhoU/PhoR interaction with genetic analysis. Using protein modeling and docking methods, we show an interaction model that points to a potential mechanism for PhoU mediated signaling to PhoR to modify its activity. This model is tested with direct coupling analysis.ConclusionsThese bioinformatics tools, in combination with genetic and biochemical analysis, help to identify and test a model for phosphate signaling and may be applicable to several other systems.

【 授权许可】

Unknown   
© Gardner et al.; licensee BioMed Central Ltd. 2015. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.