Cardiotonic pill attenuates white matter and hippocampal damage via inhibiting microglial activation and downregulating ERK and p38 MAPK signaling in chronic cerebral hypoperfused rat

BMC Complementary and Alternative Medicine

Research Article

Jung-Soo Han¹  Hyung Won Kang²  Won Kyung Jeon³  Bu Yeo Kim³  Ki Mo Lee³  In Sun Lee³  Ji Hye Bang³ 

[1] Department of Biological Sciences, Konkuk University, 1 Hwayang-dong, 143-701, Gwangjin-gu, Seoul, Republic of Korea;Department of Neuropsychiatry, College of Oriental Medicine, Won-Kwang University, 570-749, Iksan, Republic of Korea;KM-Based Herbal Drug Development Group, Korea Institute of Oriental Medicine, 305-811, Daejeon, Republic of Korea;

关键词: Cardiotonic pill;    Permanent bilateral common carotid artery occlusion;    Inflammation;    Microglia;    White matter;    Hippocampus;   

DOI  :  10.1186/1472-6882-13-334

 received in 2013-08-05, accepted in 2013-11-22,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundThe cardiotonic pill (CP) is a herbal medicine composed of Salvia miltiorrhiza (SM), Panax notoginseng (PN), and Dryobalanops aromatica Gaertner (DAG) that is widely used to treat cardiovascular diseases. The present experiment was conducted to examine the effects of CP on white matter and hippocampal damage induced by chronic cerebral hypoperfusion.MethodsChronic cerebral hypoperfusion was induced in male Wistar rats by permanent bilateral common carotid artery occlusion (BCCAo). Daily oral administration of CP (200 mg/kg) began 21 days after BCCAo and continued for 42 days. The levels of microglial activation and myelin basic protein (MBP) were measured in the white matter and hippocampus of rats with chronic BCCAo, and the expression levels of mitogen-activated protein kinases (MAPKs) and inflammatory markers such as cyclooxygenase-2, interleukin-1β, and interleukin-6 were examined.ResultsMBP expression was reduced in the white matter and hippocampal regions of rats that received BCCAo. In contrast, reduced levels of MBP were not observed in BCCAo rats given CP treatments. The administration of CP alleviated microglial activation, the alteration of ERK and p38 MAPK signaling, and inflammatory mediator expression in rats with chronic BCCAo.ConclusionThese results suggest that CP may have protective effects against chronic BCCAo-induced white matter and hippocampal damage by inhibiting inflammatory processes including microglial activation and proinflammatory mediator expression, and downreguating the hyperphosphorylation of ERK and p38 MAPK signaling.

【 授权许可】

Unknown   
© Lee et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.