Development of a point-of-care-device for fast detection of periodontal pathogens

BMC Oral Health

Technical Advance

Lisa Giel¹  Jana Berthold¹  Nadine Leitschuh¹  Christoph Boehm¹  Katja Niemann¹  Liudmila Roussak¹  Dirk Kuhlmeier¹  Cornelia Gaertig²  Katja Vetter³ 

[1] Nanotechnology Unit, Department of Diagnostics, Fraunhofer Institute for Cell Therapy and Immunology, Perlickstraße 1, 04103, Leipzig, Germany;Nanotechnology Unit, Department of Diagnostics, Fraunhofer Institute for Cell Therapy and Immunology, Perlickstraße 1, 04103, Leipzig, Germany;Friedrich Schiller University, Jena, Germany;Scanbec GmbH, Bitterfeld-Wolfen, Germany;

关键词: Bacterial quantification;    Periodontitis;    Real-time PCR;    Microbial diagnostics;    Lyophilization;   

DOI  :  10.1186/s12903-015-0155-y

 received in 2015-07-08, accepted in 2015-12-17,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundA number of pathogens can cause severe destruction of the periodontal apparatus during the course of periodontitis. The aim of this work was the development of a diagnostic device for the use at the point-of-need for the detection of periodontal pathogens to enable a personalized therapy for treatment of periodontitis.MethodsThis test system is based on the polymerase chain reaction of DNA isolated from periodontal pathogens and was examined to precisely detect species-specific sequences on a rotating chip with lyophilized reagents for polymerase chain reaction. The preservation of the reagents was optimized to ensure their stability during the storage.ResultsIn the current work, we have developed a model point-of-care device and showed a proof of concept. It requires low sample volume, is timesaving and can therefore facilitate early diagnosis and treatment of periodontal diseases.ConclusionsThe developed device can provide fast diagnosis of the composition and amount of patients’ oral flora and might help to assess the stage of periodontitis infection. This can facilitate an optimization of therapeutic approaches in order to prevent some of the more serious consequences of the disease.

【 授权许可】

CC BY   
© Gaertig et al. 2015