Gene expression comparison of resistant and susceptible Atlantic salmon fry challenged with Infectious Pancreatic Necrosis virus reveals a marked contrast in immune response

BMC Genomics

Research Article

Richard K. Paley¹  Georgina S. E. Rimmer¹  David W. Verner-Jeffreys¹  Ian J. Tew¹  John B. Taggart²  Brendan J. McAndrew²  James E. Bron²  William G. Starkey²  Jacqueline H. Ireland²  Alastair Hamilton³  Jose C. Mota-Velasco³  Alan E. Tinch³  Derrick R. Guy³  Ross D. Houston⁴  Stephen C. Bishop⁴  Chris S. Haley⁴  Diego Robledo⁵  Almas A. Gheyas⁶ 

[1] Centre for Environment, Fisheries and Aquaculture Science (Cefas), DT4 8UB, Weymouth, UK;Institute of Aquaculture, School of Natural Sciences, University of Stirling, FK9 4LA, Stirling, UK;Landcatch Natural Selection Ltd., 15 Beta Centre, Stirling University Innovation Park, FK9 4NF, Stirling, UK;The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, EH25 9RG, Midlothian, UK;The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, EH25 9RG, Midlothian, UK;Departamento de Genética, Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela, Spain;The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, EH25 9RG, Midlothian, UK;Landcatch Natural Selection Ltd., 15 Beta Centre, Stirling University Innovation Park, FK9 4NF, Stirling, UK;

关键词: Salmo salar;    virus;    transcriptome;    infectious pancreatic necrosis;    disease resistance;    interferon;    innate immunity;    aquaculture;   

DOI  :  10.1186/s12864-016-2600-y

 received in 2015-12-22, accepted in 2016-03-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundInfectious Pancreatic Necrosis (IPN) is a highly contagious birnavirus disease of farmed salmonid fish, which often causes high levels of morbidity and mortality. A large host genetic component to resistance has been previously described for Atlantic salmon (Salmo salar L.), which mediates high mortality rates in some families and zero mortality in others. However, the molecular and immunological basis for this resistance is not yet fully known. This manuscript describes a global comparison of the gene expression profiles of resistant and susceptible Atlantic salmon fry following challenge with the IPN virus.ResultsSalmon fry from two IPNV-resistant and two IPNV-susceptible full sibling families were challenged with the virus and sampled at 1 day, 7 days and 20 days post-challenge. Significant viral titre was observed in both resistant and susceptible fish at all timepoints, although generally at higher levels in susceptible fish. Gene expression profiles combined with gene ontology and pathway analyses demonstrated that while a clear immune response was observed in both resistant and susceptible fish, there were striking differences between the two phenotypes. The susceptible fish showed marked up-regulation of genes related to cytokine activity and inflammatory response that evidently failed to protect against the virus. In contrast, the resistant fish demonstrated a less pronounced immune response including up-regulation of genes relating to the M2 macrophage system.ConclusionsWhile only the susceptible phenotype shows appreciable mortality levels, both resistant and susceptible fish can become infected with IPNV. Susceptible fish are characterized by a much larger, yet ineffective, immune response, largely related to cytokine and inflammatory systems. Resistant fish demonstrate a more moderate, putative macrophage-mediated inflammatory response, which may contribute to their survival.

【 授权许可】

CC BY   
© Robledo et al. 2016