Increased expression of collagens, transforming growth factor-β1, and -β3 in gluteal muscle contracture

BMC Musculoskeletal Disorders

Research Article

Bin Lu¹  Hao-Peng Li²  Xi-Jing He²  An-Jing Kang³  Chen-Guang Zhao⁴ 

[1] Department of Orthopedic Surgery, Chinese People's Armed Police Troops Shaanxi Province Corps Hospital, Xi'an, Shaanxi, P.R. China;Department of Orthopedic Surgery, The 2nd Affiliated Hospital of Medical College, Xi'an JiaoTong University, Xi'an, Shaanxi, P.R. China;Department of Pathology, The 2nd Affiliated Hospital of Medical College, Xi'an JiaoTong University, Xi'an, Shaanxi, P.R. China;Orthopedic center of Chinese PLA, Urumqi General Hospital of Lanzhou Military Region, Urumqi, Xinjiang, P.R. China;

关键词: Collagen Type;    Fibrotic Disease;    Adjacent Muscle;    Contraction Band;    Gluteal Muscle Contracture;   

DOI  :  10.1186/1471-2474-11-15

 received in 2009-10-06, accepted in 2010-01-25,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroudGluteal muscle contracture (GMC) is a multi-factor human chronic fibrotic disease of the gluteal muscle. Fibrotic tissue is characterized by excessive accumulation of collagen in the muscle's extracellular matrix. Transforming growth factor (TGF)-β1 and -β2 are thought to play an important role in fibrogenesis, while TGF-β3 is believed to have an anti-fibrotic function. We hypothesize that the expression of collagen and TGF-βs would be up-regulated in GMC patients.MethodsThe expression of collagen type I, type III and TGF-βs were studied in 23 fibrotic samples and 23 normal/control samples in GMC patients using immunohistochemistry, reverse transcription and polymerase chain reaction (RT-PCR) and western bolt analysis.ResultsCompared to the unaffected adjacent muscle, increased expression of TGF-β1 and -β3 was associated with deposition of collagen type I and type III in the fibrotic muscle of the GMC patients at the mRNA level. Strong up-regulation of these proteins in fibrotic muscle was confirmed by immunohistochemical staining and western blot analysis. TGF-β2 was not up-regulated in relation to GMC.ConclusionThis study confirmed our hypothesis that collagen types I, III, TGF-β1 and TGF-β3 were up-regulated in biopsy specimens obtained from patients with GMC. Complex interaction of TGF-β1 with profibrotic function and TGF-β3 with antifibrotic function may increase synthesis of collagens and thereby significantly contribute to the process of gluteal muscle scarring in patients with GMC.

【 授权许可】

Unknown   
© Zhao et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.