Anti-diabetic activity of aerial parts of Sarcopoterium spinosum

BMC Complementary and Alternative Medicine

Research Article

Tovit Rosenzweig¹  Uriel Elyasiyan¹  Konstantin Rozenberg¹  Nir Skalka²  Rachela Oppenheimer³  Adi Nudel⁴  Zohar Kerem⁴  Elyashiv Drori⁵ 

[1] Department of Molecular Biology, Department of Nutritional Studies, Ariel University, 40700, Ariel, Israel;Department of Molecular Biology, Department of Nutritional Studies, Ariel University, 40700, Ariel, Israel;Diabest Botanical Drugs Ltd., 40700, Ariel, Israel;Diabest Botanical Drugs Ltd., 40700, Ariel, Israel;Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel;Samaria and Jordan Rift R&D Center, 40700, Ariel, Israel;Department of Chemical Engineering, Biotechnology and Materials, Ariel University, Ariel, Israel;

关键词: Glucose uptake;    Insulin signaling;    Medicinal plant;    Sarcopoterium spinosum;    Type 2 diabetes;   

DOI  :  10.1186/s12906-017-1860-7

 received in 2017-01-22, accepted in 2017-06-26,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundSarcopoterium spinosum (S. spinosum) is used by Bedouin medicinal practitioners for the treatment of diabetes. While the anti-diabetic activity of S. spinosum root extract was validated in previous studies, the activity of aerial parts of the same plants has not been elucidated yet. The aim of this study was to clarify the glucose lowering properties of the aerial parts of the shrub.MethodsAnti-diabetic properties were evaluated by measuring the activity of carbohydrate digesting enzymes, glucose uptake into 3 T3-L1 adipocytes, and insulin secretion. Insulin signaling cascade was followed in L6 myotubes using Western blot and PathScan analysis.ResultsActivity of α-amylase and α-glucosidase was inhibited by extracts of all S. spinosum organs. Basal and glucose-induced insulin secretion was measured in Min6 cells and found to be enhanced as well. Glucose uptake was induced by all S. spinosum extracts, with roots found to be the most effective and fruits the least.The effect of S. spinosum on Akt phosphorylation was minor compared to insulin effect. However, GSK3β and PRAS40, which are downstream elements of the insulin cascade, were found to be highly phosphorylated by S. spinosum extracts. Inhibition of PI3K and Akt, but not AMPK and ERK, abrogated the induction of glucose uptake by the aerial parts of the shrub.ConclusionThe aerial organs of S. spinosum have anti-diabetic properties and may be used as a basis for the development of dietary supplements or to identify new agents for the treatment of type 2 diabetes.

【 授权许可】

CC BY   
© The Author(s). 2017