The MDCK variety pack: choosing the right strain

BMC Cell Biology

Commentary

Andrew D Chalmers¹  Joseph D Dukes¹  Paul Whitley¹ 

[1] Department of Biology and Biochemistry, Centre for Regenerative Medicine, University of Bath, Bath, UK;

关键词: MDCK Cell;    Tight Junction Protein;    Epithelial Junction;    Epithelial Cell Polarity;    MDCK Cell Line;   

DOI  :  10.1186/1471-2121-12-43

 received in 2011-08-16, accepted in 2011-10-07,  发布年份 2011

来源: Springer

【 摘 要 】

The MDCK cell line provides a tractable model for studying protein trafficking, polarity and junctions (tight, adherens, desmosome and gap) in epithelial cells. However, there are many different strains of MDCK cells available, including the parental line, MDCK I, MDCK II, MDCK.1, MDCK.2, superdome and supertube, making it difficult for new researchers to decide which strain to use. Furthermore, there is often inadequate reporting of strain types and where cells were obtained from in the literature. This review aims to provide new researchers with a guide to the different MDCK strains and a directory of where they can be obtained. We also hope to encourage experienced researchers to report the stain and origin of their MDCK cells.

【 授权许可】

Unknown   
© Dukes et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.