Network analysis of microRNA and mRNA seasonal dynamics in a highly plastic sensorimotor neural circuit

BMC Genomics

Research Article

Eliot A. Brenowitz¹  David J. Perkel²  Nivretta M. Thatra³  Tracy A. Larson⁴  James W. MacDonald⁵  Theo K. Bammler⁵  Agata Budzillo⁶  William E. Wood⁷  Karin L. Lent⁸  Melissa L. Caras⁹ 

[1] Department of Biology, University of Washington, 98195, Seattle, WA, USA;Department of Biology, University of Washington, 98195, Seattle, WA, USA;Department of Otolaryngology, University of Washington, 98195, Seattle, WA, USA;Department of Biology, University of Washington, 98195, Seattle, WA, USA;Department of Psychology, University of Washington, 98195, Seattle, WA, USA;Department of Biology, University of Washington, 98195, Seattle, WA, USA;Present Address: Basic Sciences Division, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave N, 98109, Seattle, WA, USA;Department of Environmental and Occupational Health Sciences, University of Washington, 98195, Seattle, WA, USA;Department of Otolaryngology, University of Washington, 98195, Seattle, WA, USA;Graduate Program in Neuroscience, University of Washington, 98195, Seattle, WA, USA;Department of Otolaryngology, University of Washington, 98195, Seattle, WA, USA;Graduate Program in Neuroscience, University of Washington, 98195, Seattle, WA, USA;Present address: Centre National de la Recherche Scientifique, Laboratoire de Neurophysique et Physiologie, UMR 8119, Université Paris Descartes, 45, rue des Saints Pères, 75006, Paris, France;Department of Psychology, University of Washington, 98195, Seattle, WA, USA;Department of Psychology, University of Washington, 98195, Seattle, WA, USA;Graduate Program in Neuroscience, University of Washington, 98195, Seattle, WA, USA;Present address: Center for Neural Science, New York University, 4 Washington Place, 10003, New York, NY, USA;

关键词: microRNA;    miR–mRNA network;    Adult neurogenesis;    Seasonal plasticity;    Sex steroids;    Testosterone;    Photoperiod;    Songbird;    Bird song;   

DOI  :  10.1186/s12864-015-2175-z

 received in 2015-07-01, accepted in 2015-10-31,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAdult neurogenesis and the incorporation of adult-born neurons into functional circuits requires precise spatiotemporal coordination across molecular networks regulating a wide array of processes, including cell proliferation, apoptosis, neurotrophin signaling, and electrical activity. MicroRNAs (miRs) - short, non-coding RNA sequences that alter gene expression by post-transcriptional inhibition or degradation of mRNA sequences - may be involved in the global coordination of such diverse biological processes. To test the hypothesis that miRs related to adult neurogenesis and related cellular processes are functionally regulated in the nuclei of the avian song control circuit, we used microarray analyses to quantify changes in expression of miRs and predicted target mRNAs in the telencephalic nuclei HVC, the robust nucleus of arcopallium (RA), and the basal ganglia homologue Area X in breeding and nonbreeding Gambel’s white-crowned sparrows (Zonotrichia leucophrys gambelli).ResultsWe identified 46 different miRs that were differentially expressed across seasons in the song nuclei. miR-132 and miR-210 showed the highest differential expression in HVC and Area X, respectively. Analyzing predicted mRNA targets of miR-132 identified 33 candidate target genes that regulate processes including cell cycle control, calcium signaling, and neuregulin signaling in HVC. Likewise, miR-210 was predicted to target 14 mRNAs differentially expressed across seasons that regulate serotonin, GABA, and dopamine receptor signaling and inflammation.ConclusionsOur results identify potential miR–mRNA regulatory networks related to adult neurogenesis and provide opportunities to discover novel genetic control of the diverse biological processes and factors related to the functional incorporation of new neurons to the adult brain.

【 授权许可】

CC BY   
© Larson et al. 2015