Induction of apoptosis and oxidative stress in estrogen receptor-negative breast cancer, MDA-MB231 cells, by ethanolic mango seed extract

BMC Complementary and Alternative Medicine

Research Article

Mohamed Elwathig Saeed Mirghani¹  Abdulkarim Sabo Mohammed²  Al-Shwyeh Hussah Abdullah²  Abdullah Rasedee³  Mothanna Sadiq Al-Qubaisi⁴ 

[1] Department of Biotechnology Engineering Faculty of Engineering, International Islamic University Malaysia (IIUM), P.O. BOX 10, 50728, Kuala Lumpur, Malaysia;Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;

关键词: MDA-MB231 cells;    Breast cancer;    Apoptosis;    Oxidative stress;    Mango kernel;   

DOI  :  10.1186/s12906-015-0575-x

 received in 2014-11-12, accepted in 2015-02-21,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundIn this study, the effect of mango kernel extract in the induction of apoptosis of the breast cancer (MDA-MB-231) cell line was examined. This is an attempt to discover alternatives to current therapeutic regimes in the treatment of breast cancers.MethodsThe pro-apoptotic markers, Bax, cytochrome c, caspases-. -8 and −9, and anti-apoptotic markers, Bcl-2, p53 and glutathione were determined in MDA-MB231 cells treated for 12 and 24 h with mango kernel extract.ResultsThe results showed that the extract produced a time- and dose-dependent increases in pro-apoptotic proteins and oxidative stress markers with a corresponding decrease in anti-apoptotic markers.ConclusionsBased on the findings, mango kernel extract modulates redox balance in MDA-MB-231 breast cancer cells with a tendency for apoptotic cell death. The changes observed in this study may collectively underlie the basis for the cell death induced in MDA-MB-231 breast cancer cells by mango kernel extract. Thus, mango kernel extract has potential to be developed into an antibreast cancer mixture, and hence these results are worth studying further.

【 授权许可】

CC BY   
© Abdullah et al.; licensee BioMed Central. 2015