Prevalence and types of high-risk human papillomaviruses in head and neck cancers from Bangladesh

BMC Cancer

Research Article

Ahmed H. Chowdhury¹  Anwar Sadat²  Shahed A. Jinnah³  Aminul I. Khan⁴  Mushfiq H. Shaikh⁵  Newell W. Johnson⁶  Daniel T. W. Clarke⁷  Nigel A. J. McMillan⁷  Alfred K. Lam⁸  Vinod Gopalan⁸ 

[1] Department of Neuro-medicine, Dhaka Medical College and Hospital, 1000, Dhaka, Bangladesh;Department of Oral and Maxillo-facial Surgery, Dhaka Dental College & Hospital, 1216, Dhaka, Bangladesh;Department of Pathology, Dhaka Medical College and Hospital, 1000, Dhaka, Bangladesh;Department of Pathology, National Medical College and Hospital, 1100, Dhaka, Bangladesh;School of Dentistry and Oral Health, Griffith University, 4222, Gold Coast, QLD, Australia;School of Medical Science, Griffith University, 4222, Gold Coast, QLD, Australia;Understanding Chronic Conditions Program, Menzies Health Institute Queensland, 4222, Gold Coast, QLD, Australia;School of Dentistry and Oral Health, Griffith University, 4222, Gold Coast, QLD, Australia;Understanding Chronic Conditions Program, Menzies Health Institute Queensland, 4222, Gold Coast, QLD, Australia;Dental Institute, King’s College London, London, UK;Menzies Health Institute Queensland and School of Dentistry and Oral Health, Griffith University, Building G40, Room 9.16, Gold Coast Campus, 4222, Gold Coast, QLD, Australia;School of Medical Science, Griffith University, 4222, Gold Coast, QLD, Australia;Understanding Chronic Conditions Program, Menzies Health Institute Queensland, 4222, Gold Coast, QLD, Australia;School of Medicine, Griffith University, 4222, Gold Coast, QLD, Australia;

关键词: Head and neck squamous cell carcinoma (HNSCC);    Human papillomavirus (HPV);    Nested polymerase chain reaction (PCR);    Immunohistochemistry (IHC);    South Asia;    Bangladesh;   

DOI  :  10.1186/s12885-017-3789-0

 received in 2017-05-10, accepted in 2017-11-15,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThere is a dramatic rise in the incidence of Human papillomavirus (HPV) – associated head and neck squamous cell carcinoma (HNSCC) in the world, with considerable variation by geography, gender and ethnicity. Little is known about the situation in Bangladesh, where tobacco- and areca nut-related head and neck cancers (HNCs) are the most common cancers in men. We aimed to determine the prevalence of HPV in HNSCC in Bangladesh and to explore the possible value of cell cycle markers in clinical diagnostic settings.MethodsOne hundred and ninety six archival HNSCC tissue samples were analysed for the presence of HPV DNA. The DNA quality was assured, and then amplified using a nested PCR approach. The typing of HPV was performed by automated DNA sequencing. Cellular markers p53, Cyclin D1 and pRb were tested on all samples by immunohistochemistry (IHC), as well as p16 as a putative surrogate for the detection of HPV.ResultsHPV DNA was detected in 36/174 (~21%) samples: 36% of cancers from the oropharynx; 31% of oral cancers, and 22% from the larynx. HPV-16 was most common, being present in 33 samples, followed by HPV-33 (2 samples) and HPV-31 (1 sample). Twenty-eight out of 174 samples were positive for p16, predominantly in HPV-positive tissues (p < 0.001). No statistically significant association was observed between the cellular markers and HPV DNA positive cases. However, p16 positivity had excellent predictive value for the presence of HPV by PCR.ConclusionThere is a significant burden of HPV-associated HNSCC in Bangladesh, particularly in the oropharynx but also in oral and laryngeal cancers. Whilst a combination of PCR-based DNA detection and p16 IHC is useful, the latter has excellent specificity, acceptable sensitivity and good predictive value for carriage of HPV in this population and should be used for prognostic evaluation and treatment planning of all HNSCC patients in South Asia, as in the Western world.

【 授权许可】

CC BY   
© The Author(s). 2017