Transcriptional regulation of human sperm-associated antigen 16 gene by S-SOX5

BMC Molecular Biology

Research Article

Qiaoling Zhang¹  Wei Li²  Zhibing Zhang²  Yanwei Li³  Junpin Liu⁴  Chaofan Shuang⁵  Shizheng Song⁵  Yunhao Liu⁵  Jie Min⁵  Ling Zhang⁶ 

[1] Central China Normal University, 430000, Wuhan, Hubei, China;Department of Obstetrics and Gynecology, Virginia Commonwealth University, 23298, Richmond, VA, USA;Department of Obstetrics and Gynecology, Virginia Commonwealth University, 23298, Richmond, VA, USA;Department of Computer Science, Wellesley College, 02481-5701, Wellesley, MA, USA;Department of Obstetrics and Gynecology, Virginia Commonwealth University, 23298, Richmond, VA, USA;Wuhan Hospital for the Prevention and Treatment of Occupational Diseases, 430000, Wuhan, Hubei, China;School of Public Health, Wuhan University of Science and Technology, 430065, Wuhan, Hubei, China;School of Public Health, Wuhan University of Science and Technology, 430065, Wuhan, Hubei, China;Department of Obstetrics and Gynecology, Virginia Commonwealth University, 23298, Richmond, VA, USA;

关键词: S-SOX5;    SPAG16L;    Transcriptional regulation;    Central apparatus;    Cilia;   

DOI  :  10.1186/s12867-017-0082-3

 received in 2016-10-21, accepted in 2017-01-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe mammalian sperm-associated antigen 16 gene (Spag16) uses alternative promoters to produce two major transcript isoforms (Spag16L and Spag16S) and encode proteins that are involved in the cilia/flagella formation and motility. In silico analysis of both mouse and human SPAG16L promoters reveals the existence of multiple putative SOX5 binding sites. Given that the SOX5 gene encodes a 48-kDa transcription factor (S-SOX5) and the presence of putative SOX5 binding sites at the SPAG16L promoter, regulation of SPAG16L expression by S-SOX5 was studied in the present work.ResultsS-SOX5 activated human SPAG16L promoter activity in the human bronchial epithelia cell line BEAS-2B cells. Mutation of S-SOX5 binding sites abolished the stimulatory effect. Overexpression of S-SOX5 resulted in a significant increase in the abundance of SPAG16L transcripts whereas silencing of S-SOX5 by RNAi largely reduced the SPAG16L expression. Chromatin immunoprecipitation assays showed that S-SOX5 directly interacts with the SPAG16L promoter.ConclusionS-SOX5 regulates transcription of human SPAG16L gene via directly binding to the promoter of SPAG16L. It has been reported that expression of sperm-associated antigen 6 (SPAG6), encoding another axonemal protein, is activated by S-SOX5. Therefore, S-SOX5 may regulate formation of motile cilia/flagella through globally mediating expression of genes encoding axonemal proteins.

【 授权许可】

CC BY   
© The Author(s) 2017