Identification of the type II cytochrome cmaturation pathway in anammox bacteria by comparative genomics

BMC Microbiology

Research Article

James WA Allen¹  Christina Ferousi²  Jan TM Keltjens²  Joachim Reimann²  Huub JM Op den Camp²  Daan R Speth²  Mike SM Jetten² 

[1] Department of Biochemistry, University of Oxford, South Parks Road, OX13QU, Oxford, UK;Department of Microbiology, Institute for Water and Wetland Research, Radboud University Nijmegen, Heyendaalseweg 135, 6525, Nijmegen, AJ, the Netherlands;

关键词: c;    Anaerobic ammonium oxidizing bacteria;    ccs;    CcsA;    CcsB;    CcsX;    DsbD;    CcdA;   

DOI  :  10.1186/1471-2180-13-265

 received in 2013-06-26, accepted in 2013-11-18,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundAnaerobic ammonium oxidizing (anammox) bacteria may contribute up to 50% to the global nitrogen production, and are, thus, key players of the global nitrogen cycle. The molecular mechanism of anammox was recently elucidated and is suggested to proceed through a branched respiratory chain. This chain involves an exceptionally high number of c-type cytochrome proteins which are localized within the anammoxosome, a unique subcellular organelle. During transport into the organelle the c-type cytochrome apoproteins need to be post-translationally processed so that heme groups become covalently attached to them, resulting in mature c-type cytochrome proteins.ResultsIn this study, a comparative genome analysis was performed to identify the cytochrome c maturation system employed by anammox bacteria. Our results show that all available anammox genome assemblies contain a complete type II cytochrome c maturation system.ConclusionsOur working model suggests that this machinery is localized at the anammoxosome membrane which is assumed to be the locus of anammox catabolism. These findings will stimulate further studies in dissecting the molecular and cellular basis of cytochrome c biogenesis in anammox bacteria.

【 授权许可】

Unknown   
© Ferousi et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.