Chemopreventive and immunomodulatory effects of Murraya koenigii aqueous extract on 4T1 breast cancer cell-challenged mice

BMC Complementary and Alternative Medicine

Research Article

Boon Kee Beh¹  Nadiah Abu²  Nurul Elyani Mohamad²  Noorjahan Banu Alitheen²  Huynh Ky³  Swee Keong Yeap⁴  Siamak Ebrahimi⁴  Hamidah Mohd Yusof⁴  Sheau Wei Tan⁴  Wan Yong Ho⁵ 

[1] Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia;Department of Agriculture Genetics and Breeding, College of Agriculture and Applied Biology, Cantho University, 3/2 Street, Can Tho City, Vietnam;Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia;School of Biomedical Sciences, The University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor, Malaysia;

关键词: Anti-inflammation;    Chemopreventive;    Curry leaves;    Murraya koenigii;    4T1 cells;   

DOI  :  10.1186/s12906-015-0832-z

 received in 2015-06-19, accepted in 2015-08-26,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundThe progression of breast cancer is increasing at an alarming rate, particularly in western countries. Meanwhile, the lower incidence in Asian countries could be attributed to the heavy incorporation of green leaves vegetables or spices in their diets. Murraya koenigii (MK) or often times known as curry leaves are common spice used mostly in tropical countries. Anti-inflammatory and chemopreventive effects of MK aqueous extract on 4T1 breast cancer cell-challenged mice were evaluated.MethodsHerein, cytotoxic activity of MK was first tested on 4T1 cells in vitroby MTT assay. Then, in vivo chemopreventive study was conducted where mice were fed with extracts prior to and after inducing the tumor (inoculation). Tumor size was monitored post-4T1 inoculation. At the end of experiment, histopathology of tumor sections, T cell immunophenotyping, tumor nitric oxide level, serum cytokine level and qPCR analysis on expression of iNOS, iCAM, NF-kB and c-MYC were performed.ResultsMK reduced the tumors’ size and lung metastasis aside from inhibited the viability of 4T1 cells in vitro. Furthermore, it decreased the level of nitric oxide and inflammation-related cytokines and genes, including iNOS, iCAM, NF-kB and c-MYC.ConclusionThe results propose that, MK managed to inhibit the progression of tumor via immunostimulatory effect and inflammatory reaction within the tumor samples. This suggests that MKconsumption could be a savior in the search of new chemopreventive agents.

【 授权许可】

CC BY   
© Yeap et al. 2015

【 图 表 】

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