Non-typeable Haemophilus influenzae and Streptococcus pneumoniaeas primary causes of acute otitis media in colombian children: a prospective study

BMC Infectious Diseases

Research Article

Pio Lopez¹  Mercedes A Zapata¹  Alexandra Sierra¹  Beatriz Vanegas¹  Maria M Castrejon²  Rodrigo DeAntonio²  Romulo E Colindres³  William P Hausdorff⁴ 

[1] Centro de Estudios en Infectologia Pediatrica CEIP, Cali, Colombia;GlaxoSmithKline Biologicals, Panama City, Panama;GlaxoSmithKline Biologicals, Rio de Janeiro, Brazil;GlaxoSmithKline Biologicals, Wavre, Belgium;

关键词: Acute Otitis Medium;    Pneumococcal Conjugate Vaccine;    Pneumococcal Serotypes;    Recurrent Acute Otitis Medium;    Acute Otitis Medium Episode;   

DOI  :  10.1186/1471-2334-11-4

 received in 2010-09-30, accepted in 2011-01-05,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundAcute otitis media (AOM) is one of the most frequently encountered bacterial infections in children aged < 5 years; Streptococcus pneumoniae (S. pneumoniae) and non-typeable Haemophilus influenzae (NTHi) are historically identified as primary AOM causes. Nevertheless, recent data on bacterial pathogens causing AOM in Latin America are limited. This prospective study aimed to identify and characterize bacterial etiology and serotypes of AOM cases including antimicrobial susceptibility in < 5 year old Colombian children.MethodsFrom February 2008 to January 2009, children ≥3 months and < 5 years of age presenting with AOM and for whom a middle ear fluid (MEF) sample was available were enrolled in two medical centers in Cali, Colombia. MEF samples were collected either by tympanocentesis procedure or spontaneous otorrhea swab sampling. Bacteria were identified using standard laboratory methods, and antimicrobial resistance testing was performed based on the 2009 Clinical and Laboratory Standards Institute (CLSI) criteria. Most of the cases included in the study were sporadic in nature.ResultsOf the 106 enrolled children, 99 were included in the analysis. Bacteria were cultured from 62/99 (63%) of samples with S. pneumoniae, H. influenzae, or S. pyogenes. The most commonly isolated bacteria were H. influenzae in 31/99 (31%) and S. pneumoniae in 30/99 (30%) of samples. The majority of H. influenzae episodes were NTHi (27/31; 87%). 19F was the most frequently isolated pneumococcal serotype (10/30; 33%). Of the 30 S. pneumoniae positive samples, 8/30 (27%) were resistant to tetracycline, 5/30 (17%) to erythromycin and 8/30 (27%) had intermediate resistance to penicillin. All H. influenzae isolates tested were negative to beta-lactamase.ConclusionsNTHi and S. pneumoniae are the leading causes of AOM in Colombian children. A pneumococcal conjugate vaccine that prevents both pathogens could be useful in maximizing protection against AOM.

【 授权许可】

Unknown   
© Sierra et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.