Comparison of koala LPCoLN and human strains of Chlamydia pneumoniae highlights extended genetic diversity in the species

BMC Genomics

Research Article

Kelley M Hovis¹  Jose A Carrasco¹  Patrik M Bavoil¹  Garry SA Myers²  Candice M Mitchell³  Peter Timms³ 

[1] Department of Microbial Pathogenesis, University of Maryland, 21201, Baltimore, Maryland, USA;Institute for Genome Sciences, University of Maryland, 21201, Baltimore, Maryland, USA;Institute of Health and Biomedical Innovation, Faculty of Science and Technology, Queensland University of Technology, Kelvin Grove, 4059, Queensland, Australia;

关键词: Human Isolate;    Chlamydial Species;    Orotate Phosphoribosyl Transferase;    Extrachromosomal Element;    Respiratory Isolate;   

DOI  :  10.1186/1471-2164-11-442

 received in 2009-11-06, accepted in 2010-07-21,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundChlamydia pneumoniae is a widespread pathogen causing upper and lower respiratory tract infections in addition to a range of other diseases in humans and animals. Previous whole genome analyses have focused on four essentially clonal (> 99% identity) C. pneumoniae human genomes (AR39, CWL029, J138 and TW183), providing relatively little insight into strain diversity and evolution of this species.ResultsWe performed individual gene-by-gene comparisons of the recently sequenced C. pneumoniae koala genome and four C. pneumoniae human genomes to identify species-specific genes, and more importantly, to gain an insight into the genetic diversity and evolution of the species. We selected genes dispersed throughout the chromosome, representing genes that were specific to C. pneumoniae, genes with a demonstrated role in chlamydial biology and/or pathogenicity (n = 49), genes encoding nucleotide salvage or amino acid biosynthesis proteins (n = 6), and extrachromosomal elements (9 plasmid and 2 bacteriophage genes).ConclusionsWe have identified strain-specific differences and targets for detection of C. pneumoniae isolates from both human and animal origin. Such characterisation is necessary for an improved understanding of disease transmission and intervention.

【 授权许可】

Unknown   
© Mitchell et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.