Tumor vaccine composed of C-class CpG oligodeoxynucleotides and irradiated tumor cells induces long-term antitumor immunity

BMC Immunology

Research Article

Barbara Jezersek Novakovic¹  Petra Cerkovnik²  Srdjan Novakovic²  Vida Stegel² 

[1] Department of Medical Oncology, Institute of Oncology Ljubljana, 1000, Zaloska 2, Ljubljana, Slovenia;Department of Molecular Diagnostics, Institute of Oncology Ljubljana, 1000, Zaloska 2, Ljubljana, Slovenia;

关键词: Antitumor Immunity;    Peritoneal Lavage;    Viable Tumor Cell;    Tumor Vaccine;    Tumor Challenge;   

DOI  :  10.1186/1471-2172-11-45

 received in 2010-03-03, accepted in 2010-09-13,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundAn ideal tumor vaccine should activate both effector and memory immune response against tumor-specific antigens. Beside the CD8+ T cells that play a central role in the generation of a protective immune response and of long-term memory, dendritic cells (DCs) are important for the induction, coordination and regulation of the adaptive immune response. The DCs can conduct all of the elements of the immune orchestra and are therefore a fundamental target and tool for vaccination. The present study was aimed at assessing the ability of tumor vaccine composed of C-class CpG ODNs and irradiated melanoma tumor cells B16F1 followed by two additional injections of CpG ODNs to induce the generation of a functional long-term memory response in experimental tumor model in mice (i.p. B16F1).ResultsIt has been shown that the functional memory response in vaccinated mice persists for at least 60 days after the last vaccination. Repeated vaccination also improves the survival of experimental animals compared to single vaccination, whereas the proportion of animals totally protected from the development of aggressive i.p. B16F1 tumors after vaccination repeated three times varies between 88.9%-100.0%. Additionally, the long-term immune memory and tumor protection is maintained over a prolonged period of time of at least 8 months. Finally, it has been demonstrated that following the vaccination the tumor-specific memory cells predominantly reside in bone marrow and peritoneal tissue and are in a more active state than their splenic counterparts.ConclusionsIn this study we demonstrated that tumor vaccine composed of C-class CpG ODNs and irradiated tumor cells followed by two additional injections of CpG ODNs induces a long-term immunity against aggressive B16F1 tumors.

【 授权许可】

Unknown   
© Cerkovnik et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.