Genome of Epinotia aporema granulovirus (EpapGV), a polyorganotropic fast killing betabaculovirus with a novel thymidylate kinase gene

BMC Genomics

Research Article

María Leticia Ferrelli¹  Santiago Haase¹  Víctor Romanowski¹  Marina Elizabeth Biedma²  Ricardo Salvador³  Alicia Sciocco-Cap⁴  Marcelo Facundo Berretta⁴  Pablo Daniel Ghiringhelli⁵ 

[1] Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, La Plata, Argentina;Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, La Plata, Argentina;INSERM U748, Institut de Virologie, Faculté de Médecine, Université de Strasbourg, 3 rue Koeberlé, 67000, Strasbourg, France;Instituto de Biotecnología y Biología Molecular, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, CONICET, La Plata, Argentina;Instituto de Microbiología y Zoología Agrícola, INTA, Castelar, Argentina;Instituto de Microbiología y Zoología Agrícola, INTA, Castelar, Argentina;Laboratorio de Ingeniería Genética y Biología Celular y Molecular - Area Virosis de Insectos, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina;

关键词: Thymidine Kinase;    Core Gene;    White Spot Syndrome Virus;    African Swine Fever Virus;    Early Promoter;   

DOI  :  10.1186/1471-2164-13-548

 received in 2011-11-23, accepted in 2012-09-22,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundEpinotia aporema (Lepidoptera: Tortricidae) is an important pest of legume crops in South America. Epinotia aporema granulovirus (EpapGV) is a baculovirus that causes a polyorganotropic infection in the host larva. Its high pathogenicity and host specificity make EpapGV an excellent candidate to be used as a biological control agent.ResultsThe genome of Epinotia aporema granulovirus (EpapGV) was sequenced and analyzed. Its circular double-stranded DNA genome is 119,082 bp in length and codes for 133 putative genes. It contains the 31 baculovirus core genes and a set of 19 genes that are GV exclusive. Seventeen ORFs were unique to EpapGV in comparison with other baculoviruses. Of these, 16 found no homologues in GenBank, and one encoded a thymidylate kinase. Analysis of nucleotide sequence repeats revealed the presence of 16 homologous regions (hrs) interspersed throughout the genome. Each hr was characterized by the presence of 1 to 3 clustered imperfect palindromes which are similar to previously described palindromes of tortricid-specific GVs. Also, one of the hrs (hr4) has flanking sequences suggestive of a putative non-hr ori. Interestingly, two more complex hrs were found in opposite loci, dividing the circular dsDNA genome in two halves. Gene synteny maps showed the great colinearity of sequenced GVs, being EpapGV the most dissimilar as it has a 20 kb-long gene block inversion. Phylogenetic study performed with 31 core genes of 58 baculoviral genomes suggests that EpapGV is the baculovirus isolate closest to the putative common ancestor of tortricid specific betabaculoviruses.ConclusionsThis study, along with previous characterization of EpapGV infection, is useful for the better understanding of the pathology caused by this virus and its potential utilization as a bioinsecticide.

【 授权许可】

Unknown   
© Ferrelli et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.