期刊论文详细信息
BMC Microbiology
New insights in Staphylococcus pseudintermedius pathogenicity: antibiotic-resistant biofilm formation by a human wound-associated strain
Research Article
Simone Guarnieri1  Vincenzo Savini2  Valentina Crocetta3  Arianna Pompilio3  Giovanni Di Bonaventura3  Serena De Nicola3  Edoardo Carretto4 
[1] Center for Research on Ageing, “G. d’Annunzio” University Foundation, Chieti, Italy;Department of Neuroscience and Imaging, “G. d’Annunzio” University, Chieti, Italy;Clinical Microbiology and Virology, “Spirito Santo” Hospital, Pescara, Italy;Department of Medical, Oral, and Biotechnological Sciences, “G. d’Annunzio” University, Via Vestini 31, 66100, Chieti, Italy;Center for Research on Ageing, “G. d’Annunzio” University Foundation, Chieti, Italy;IRCCS Arcispedale, “Santa Maria Nuova”, Reggio Emilia, Italy;
关键词: Staphylococcus pseudintermedius;    zoonotic infection;    Biofilm formation;    Wound infection;    Antibiotic-resistance;   
DOI  :  10.1186/s12866-015-0449-x
 received in 2014-10-20, accepted in 2015-05-15,  发布年份 2015
来源: Springer
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【 摘 要 】

BackgroundStaphylococcus pseudintermedius is an opportunistic pathogen recognized as the leading cause of skin, ear, and post-operative bacterial infections in dogs and cats. Zoonotic infections have also recently been reported causing endocarditis, infection of surgical wounds, rhinosinusitis, and catheter-related bacteremia. The aim of the present study is to evaluate, for the first time, the pathogenic potential of S. pseudintermedius isolated from a human infection. To this end, strain DSM 25713, which was recently isolated from a wound of a leukemic patient who underwent a bone marrow transplantation, was investigated for biofilm formation and antibiotic-resistance under conditions relevant for wound infection.ResultsThe effect of pH (5.5, 7.1, and 8.7) and the presence of serum (diluted at 1:2, 1:10, and 1:100) on biofilm formation was assessed through a crystal violet assay. The presence of serum significantly reduced the ability to form biofilm, regardless of the pH value tested. In vitro activity of eight antibiotics against biofilm formation and mature 48 h-old biofilms was comparatively assessed by crystal violet assay and viable cell count, respectively. Antibiotics at sub-inhibitory concentrations reduced biofilm formation in a dose-dependent manner, although cefoxitin was the most active, causing a significant reduction already at 1/8xMIC. Rifampicin showed the highest activity against preformed biofilms (MBEC90: 2xMIC). None of the antibiotics completely eradicated the preformed biofilms, regardless of tested concentrations. Confocal and electron microscopy analyses of mature biofilm revealed a complex “mushroom-like” architecture consisting of microcolonies embedded in a fibrillar extracellular matrix.ConclusionsFor the first time, our results show that human wound-associated S. pseudintermedius is able to form inherently antibiotic-resistant biofilms, suggestive of its pathogenic potential, and consistent with recent reports of zoonotic infections.

【 授权许可】

Unknown   
© Pompilio et al.; licensee BioMed Central. 2015. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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