Automatic analysis of the 3-D microstructure of fruit parenchyma tissue using X-ray micro-CT explains differences in aeration

BMC Plant Biology

Methodology Article

Metadel Abera¹  Pieter Verboven¹  Dennis Cantre¹  Els Herremans¹  Bart M. Nicolaï²  Bert E. Verlinden³  Martine Wevers⁴ 

[1] BIOSYST-MeBioS, KU Leuven, Willem de Croylaan 42, 3001, Leuven, Belgium;BIOSYST-MeBioS, KU Leuven, Willem de Croylaan 42, 3001, Leuven, Belgium;Flanders Centre of Postharvest Technology, Willem de Croylaan 42, 3001, Leuven, Belgium;Flanders Centre of Postharvest Technology, Willem de Croylaan 42, 3001, Leuven, Belgium;MTM, KU Leuven, Kasteelpark Arenberg 44, 3001, Leuven, Belgium;

关键词: Image analysis;    Apple;    Pear;    Diffusion;    Oxygen;    Gas space;    Mathematical model;    Tomography;   

DOI  :  10.1186/s12870-015-0650-y

 received in 2015-05-11, accepted in 2015-10-21,  发布年份 2015

来源: Springer

【 摘 要 】

Background3D high-resolution X-ray imaging methods have emerged over the last years for visualising the anatomy of tissue samples without substantial sample preparation. Quantitative analysis of cells and intercellular spaces in these images has, however, been difficult and was largely based on manual image processing. We present here an automated procedure for processing high-resolution X-ray images of parenchyma tissues of apple (Malus × domestica Borkh.) and pear (Pyrus communis L.) as a rapid objective method for characterizing 3D plant tissue anatomy at the level of single cells and intercellular spaces.ResultsWe isolated neighboring cells in 3D images of apple and pear cortex tissues, and constructed a virtual sieve to discard incorrectly segmented cell particles or unseparated clumps of cells. Void networks were stripped down until their essential connectivity features remained. Statistical analysis of structural parameters showed significant differences between genotypes in the void and cell networks that relate to differences in aeration properties of the tissues.ConclusionsA new model for effective oxygen diffusivity of parenchyma tissue is proposed that not only accounts for the tortuosity of interconnected voids, but also for significant diffusion across cells where the void network is not connected. This will significantly aid interpretation and analysis of future tissue aeration studies. The automated image analysis methodology will also support pheno- and genotyping studies where the 3D tissue anatomy plays a role.

【 授权许可】

CC BY   
© Herremans et al. 2015

【 图 表 】

12864_2017_4320_Article_IEq1.gif

12864_2017_3777_Article_IEq12.gif

12864_2017_3781_Article_IEq8.gif

12864_2017_3781_Article_IEq7.gif

12864_2017_3733_Article_IEq50.gif

12864_2017_3798_Article_IEq2.gif

12864_2017_3777_Article_IEq7.gif

12870_2015_650_Article_IEq1.gif