Overexpression of wheat ferritin gene TaFER-5B enhances tolerance to heat stress and other abiotic stresses associated with the ROS scavenging

BMC Plant Biology

Research Article

Zhongfu Ni¹  Qixin Sun¹  Yue Zhao¹  Xuejun Tian¹  Xinshan Zang¹  Liyuan Zhang¹  Mingming Xin¹  Yingyin Yao¹  Zhenshan Liu¹  Huiru Peng¹  Fei Wang¹  Zhaorong Hu¹  Xiaoli Geng¹ 

[1] State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization (MOE), Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, NO.2 Yuanmingyuan Xi Road, Haidian District, 100193, Beijing, China;

关键词: TaFER-5B;    Heat stress;    Abiotic stress;    Ferritin-lacking mutant;    Wheat;    Arabidopsis;   

DOI  :  10.1186/s12870-016-0958-2

 received in 2016-06-08, accepted in 2016-12-20,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundThe yield of wheat (Triticum aestivum L.), an important crop, is adversely affected by heat stress in many regions of the world. However, the molecular mechanisms underlying thermotolerance are largely unknown.ResultsA novel ferritin gene, TaFER, was identified from our previous heat stress-responsive transcriptome analysis of a heat-tolerant wheat cultivar (TAM107). TaFER was mapped to chromosome 5B and named TaFER-5B. Expression pattern analysis revealed that TaFER-5B was induced by heat, polyethylene glycol (PEG), H2O2 and Fe-ethylenediaminedi(o-hydroxyphenylacetic) acid (Fe-EDDHA). To confirm the function of TaFER-5B in wheat, TaFER-5B was transformed into the wheat cultivar Jimai5265 (JM5265), and the transgenic plants exhibited enhanced thermotolerance. To examine whether the function of ferritin from mono- and dico-species is conserved, TaFER-5B was transformed into Arabidopsis, and overexpression of TaFER-5B functionally complemented the heat stress-sensitive phenotype of a ferritin-lacking mutant of Arabidopsis. Moreover, TaFER-5B is essential for protecting cells against heat stress associated with protecting cells against ROS. In addition, TaFER-5B overexpression also enhanced drought, oxidative and excess iron stress tolerance associated with the ROS scavenging. Finally, TaFER-5B transgenic Arabidopsis and wheat plants exhibited improved leaf iron content.ConclusionsOur results suggest that TaFER-5B plays an important role in enhancing tolerance to heat stress and other abiotic stresses associated with the ROS scavenging.

【 授权许可】

CC BY   
© The Author(s). 2017