Yiguanjian decoction and its ingredients inhibit angiogenesis in carbon tetrachloride-induced cirrhosis mice

BMC Complementary and Alternative Medicine

Research Article

Ming-Yu Sun¹  Ya-Ning Zhou¹  Wen-Wei Fu¹  Hua Zhang¹  Lie-Ming Xu¹  Yong-Ping Mu¹  Bing-Bing Ning¹  Jia-Mei Chen¹  Ping Liu²  Yi-Yang Hu²  Cheng-Hai Liu²  Guang-Li Du³ 

[1]Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China

[2]Institute of Liver Diseases, Shuguang Hospital, Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China

[3]E-institute of Shanghai Municipal Education Commission, 201203, Shanghai, China

[4]Shanghai University of Traditional Chinese Medicine, 201203, Shanghai, China

关键词: Cirrhosis;    Angiogenesis;    Yiguanjian;   

DOI  :  10.1186/s12906-015-0862-6

 received in 2013-08-22, accepted in 2015-09-16,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundCirrhosis is associated with angiogenesis and disruption of hepatic vascular architecture. Yiguanjian (YGJ) decoction, a prescription from traditional Chinese medicine, is widely used for treating liver diseases. We studied whether YGJ or its ingredients (iYGJ) had an anti-angiogenic effect and explored possible mechanisms underlying this process.MethodsCirrhosis was induced with carbon tetrachloride (CCl4) (ip) in C57BL/6 mice for 6 weeks. From week 4 to week 6, cirrhotic mice were randomly divided into four groups: sorafenib-treated, YGJ-treated and iYGJ-treated mice and placebo. Serum biochemistries, hydroxyproline (Hyp) content and histopathological changes of hepatic tissues were measured as were α-smooth muscle actin (α-SMA), collagen I, CD31, vascular endothelial growth factor (VEGF), VEGF receptor (VEGFR) 2 and hypoxia-inducible factor (HIF)-1α.ResultsBoth YGJ and iYGJ improved serum biochemistries. Changes of histopathology showed that YGJ and iYGJ reduced hepatic tissue necroinflammatory and collagen fiber deposition in cirrhosis mice. Compared to the CCl4 treated animals, Hyp, α-SMA, collagen I, CD31, VEGF, VEGFR, and HIF-1α expression decreased in YGJ and iYGJ groups.ConclusionsYGJ and iYGJ inhibited liver angiogenesis in cirrhotic mice treated with CCl4 by inhibiting the HIF-1α/VEGF signaling pathway, suggesting that anti-angiogenic effects of YGJ and iYGJ are associated with improving the hepatic hypoxic microenvironment.

【 授权许可】

CC BY   
© Zhou et al. 2015