Target-site and non-target-site based resistance to the herbicide tribenuron-methyl in flixweed (Descurainia sophia L.)

BMC Genomics

Research Article

Qin Yu¹  Xuefeng Li²  Mingqi Zheng²  Qian Yang²  Wei Deng²  Lianyang Bai³ 

[1] Australian Herbicide Resistance Initiative, School of Plant Biology, University of Western Australia, 6009, Crawley, WA, Australia;Department of Applied Chemistry, College of Science, China Agricultural University, 100193, Beijing, China;Hunan Academy of Agricultural Science, 410125, Changsha, China;

关键词: Acetolactate synthase;    Cytochrome P450;    Flixweed;    Tribenuron-methyl;    Target-site based resistance;    Non-target-site based resistance;    Metabolic resistance;    RNA-Seq;   

DOI  :  10.1186/s12864-016-2915-8

 received in 2016-04-06, accepted in 2016-07-07,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundFlixweed (Descurainia sophia L.) is a troublesome and widespread broadleaf weed in winter fields in China, and has evolved high level resistance to acetolactate synthase (ALS)-inhibiting sulfonylurea herbicide tribenuron-methyl.ResultsWe identified a resistant flixweed population (N11) exhibiting 116.3-fold resistance to tribenuron-methyl relative to the susceptible population (SD8). Target-site ALS gene mutation Pro-197-Thr was identified in resistant plants. Moreover, the resistance can be reversed to 28.7-fold by the cytochrome P450 inhibitor malathion. The RNA-Sequencing was employed to identify candidate genes involved in non-target-site metabolic resistance in this population. Total 26 differentially expressed contigs were identified and eight of them (four P450s, one ABC transporter, three glycosyltransferase) verified by qRT-PCR. Consistent over-expression of the two contigs homology to CYP96A13 and ABCC1 transporter, respectively, were further qRT-PCR validated using additional plants from the resistant and susceptible populations.ConclusionsTribenuron-methyl resistance in flixweed is controlled by target-site ALS mutation and non-target-site based mechanisms. Two genes, CYP96A13 and ABCC1 transporter, could play an important role in metabolic resistance to tribenuron-methyl in the resistant flixweed population and justify further functional studies.

【 授权许可】

CC BY   
© The Author(s). 2016