In vitro membrane stabilizing, thrombolytic and antioxidant potentials of Drynaria quercifolia L., a remedial plant of the Garo tribal people of Bangladesh

BMC Complementary and Alternative Medicine

Research Article

Mahbuba Khatun¹  Farjana Rahman Chaity²  Mohammad Sharifur Rahman² 

[1] Department of Population Sciences, University of Dhaka, 1000, Dhaka, Bangladesh;Phytochemical Research Laboratory, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Dhaka, 1000, Dhaka, Bangladesh;

关键词: Drynaria quercifolia;    Membrane stabilizing;    Thrombolytic;    DPPH;    Hydrogen peroxide;    ABTS;    FRAP;    Antioxidant;    The Garo;   

DOI  :  10.1186/s12906-016-1170-5

 received in 2015-11-21, accepted in 2016-06-22,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundDrynaria quercifolia L. (Family- Polypodiaceae) is a fern grows in Bangladesh used in traditional healing by the Garo tribe of Mymensingh district. In the current study, rhizomes and fertile foliage fronds of this plant have been investigated comprehensively to assess their in vitro membrane stabilizing, thrombolytic and antioxidant properties.MethodsRhizomes and fertile foliage fronds of D. quercifolia were collected, dried, powdered and extracted with methanol. Later on, crude methanol extracts of the plant parts were fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions. The extractives were then subjected to membrane stabilizing, thrombolytic and antioxidant assays.ResultsIn membrane stabilizing assay, crude methanol extracts of rhizomes and fertile foliage fronds and their petroleum ether fractions were found to be very effective for stabilizing erythrocyte membrane in hypotonic solution. In case of thrombolytic study, crude methanol extract of rhizomes and its aqueous fraction exhibited noticeable clot lysis. However, in antioxidant assays, crude methanol extracts of the tested plant parts and their aqueous fractions exhibited potent 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide and 2, 2’-azinobis (3-ethylbenzothiazoline sulphonic acid) (ABTS) radical scavenging activity. Besides, these extractives also displayed substantial ferric reducing potential in ferric reducing antioxidant power (FRAP) assay. Crude methanol extracts of the plant parts and their aqueous fractions were also found rich in phenolics.ConclusionThis study demonstrates the medicinal potentials of D. quercifolia and justifies the local uses of it by the Garo tribal people of Bangladesh for multiple disease management.

【 授权许可】

CC BY   
© The Author(s). 2016