Ragweed (Ambrosia artemisiifolia) pollen allergenicity: SuperSAGE transcriptomic analysis upon elevated CO2 and drought stress

BMC Plant Biology

Research Article

Claudia Traidl-Hoffmann¹  Heidrun Behrendt²  Ralf Horres³  Werner Heller⁴  Feng Zhao⁴  Jörg Durner⁵  Amr El Kelish⁶  Ulrike Frank⁷  Dieter Ernst⁷  Matthias Pfeifer⁸  J Barbro Winkler⁹ 

[1] CK-CARE, Christine Kühne – Center for Allergy Research and Education, Davos, Switzerland;Institute of Environmental Medicine, UNIKA-T, Technische Universität München, Munich, Germany;Center of Allergy & Environment München (ZAUM), Technische Universität and Helmholtz Zentrum München, 85764, Neuherberg, Germany;CK-CARE, Christine Kühne – Center for Allergy Research and Education, Davos, Switzerland;GenXPro GmbH, 60438, Frankfurt am Main, Germany;Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany;Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany;Biochemical Plant Pathology, Technische Universität München, Center of Life and Food Sciences Weihenstephan, 85350, Freising-Weihenstephan, Germany;Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany;Botany Department, Faculty of Science, Suez Canal University, Ismailia, Egypt;Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany;CK-CARE, Christine Kühne – Center for Allergy Research and Education, Davos, Switzerland;Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany;Research Unit for Environmental Simulation, Helmholtz Zentrum München, German Research Center for Environmental Health, 85764, Neuherberg, Germany;

关键词: Ambrosia artemisiifolia;    Allergen;    Allergy;    CO;    Drought;    Flavonoids;    Pollen;    Ragweed;    Scanning electron microscopy;    Transcriptome;   

DOI  :  10.1186/1471-2229-14-176

 received in 2014-02-10, accepted in 2014-06-18,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundPollen of common ragweed (Ambrosia artemisiifolia) is a main cause of allergic diseases in Northern America. The weed has recently become spreading as a neophyte in Europe, while climate change may also affect the growth of the plant and additionally may also influence pollen allergenicity. To gain better insight in the molecular mechanisms in the development of ragweed pollen and its allergenic proteins under global change scenarios, we generated SuperSAGE libraries to identify differentially expressed transcripts.ResultsRagweed plants were grown in a greenhouse under 380 ppm CO2 and under elevated level of CO2 (700 ppm). In addition, drought experiments under both CO2 concentrations were performed. The pollen viability was not altered under elevated CO2, whereas drought stress decreased its viability. Increased levels of individual flavonoid metabolites were found under elevated CO2 and/or drought. Total RNA was isolated from ragweed pollen, exposed to the four mentioned scenarios and four SuperSAGE libraries were constructed. The library dataset included 236,942 unique sequences, showing overlapping as well as clear differently expressed sequence tags (ESTs). The analysis targeted ESTs known in Ambrosia, as well as in pollen of other plants. Among the identified ESTs, those encoding allergenic ragweed proteins (Amb a) increased under elevated CO2 and drought stress. In addition, ESTs encoding allergenic proteins in other plants were also identified.ConclusionsThe analysis of changes in the transcriptome of ragweed pollen upon CO2 and drought stress using SuperSAGE indicates that under global change scenarios the pollen transcriptome was altered, and impacts the allergenic potential of ragweed pollen.

【 授权许可】

Unknown   
© El Kelish et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.