Protein composition of interband regions in polytene and cell line chromosomes of Drosophila melanogaster

BMC Genomics

Research Article

Vladimir N Babenko¹  Valery F Semeshin²  Sergey A Demakov²  Elena S Belyaeva²  Igor F Zhimulev²  Tatyana Yu Vatolina² 

[1] Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 630090, Novosibirsk, Russia;Institute of Molecular and Cellular Biology, Siberian Branch of Russian Academy of Sciences, 630090, Novosibirsk, Russia;

关键词: Polytene Chromosome;    Histone Mark;    Protein Binding Site;    Open Chromatin;    Chromatin Protein;   

DOI  :  10.1186/1471-2164-12-566

 received in 2011-08-05, accepted in 2011-11-18,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundDespite many efforts, little is known about distribution and interactions of chromatin proteins which contribute to the specificity of chromomeric organization of interphase chromosomes. To address this issue, we used publicly available datasets from several recent Drosophila genome-wide mapping and annotation projects, in particular, those from modENCODE project, and compared molecular organization of 13 interband regions which were accurately mapped previously.ResultsHere we demonstrate that in interphase chromosomes of Drosophila cell lines, the interband regions are enriched for a specific set of proteins generally characteristic of the "open" chromatin (RNA polymerase II, CHRIZ (CHRO), BEAF-32, BRE1, dMI-2, GAF, NURF301, WDS and TRX). These regions also display reduced nucleosome density, histone H1 depletion and pronounced enrichment for ORC2, a pre-replication complex component. Within the 13 interband regions analyzed, most were around 3-4 kb long, particularly those where many of said protein features were present. We estimate there are about 3500 regions with similar properties in chromosomes of D. melanogaster cell lines, which fits quite well the number of cytologically observed interbands in salivary gland polytene chromosomes.ConclusionsOur observations suggest strikingly similar organization of interband chromatin in polytene chromosomes and in chromosomes from cell lines thereby reflecting the existence of a universal principle of interphase chromosome organization.

【 授权许可】

Unknown   
© Demakov et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.