Protective effects of Zhuyeqing liquor on the immune function of normal and immunosuppressed mice in vivo

BMC Complementary and Alternative Medicine

Research Article

Guo-yu Li¹  Hang-yu Wang¹  Jian Huang²  Hong-ying Gao²  Fu-zhou Sun²  Jin-hui Wang³  Li-juan An⁴  Xiao-wei Du⁴  Ying Han⁴ 

[1] School of Pharmacy, Shihezi University, 832002, Shihezi, P.R. China;School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 110016, Shenyang, P.R. China;School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, 110016, Shenyang, P.R. China;School of Pharmacy, Shihezi University, 832002, Shihezi, P.R. China;Shanxi Xinghuacun Fen Jiu Group Co. Ltd, 450000, Shanxi, P.R. China;

关键词: Zhuyeqing liquor;    Immune function;    Antioxidant effects;    Cyclophosphamide;   

DOI  :  10.1186/1472-6882-13-252

 received in 2013-03-26, accepted in 2013-09-19,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundZhuyeqing Liquor (ZYQL), a well-known Chinese traditional health liquor, has various biological properties, including anti-oxidant, anti-inflammatory, immunoenhancement and cardiovascular protective effects.MethodsThe protective effects of Zhuyeqing Liquor (ZYQL) on the immune function was investigated in vivo in normal healthy mice and immunosuppressed mice treated with Cyclophosphamide (Cy, 100 mg/kg) by intraperitoneal injection on days 4, 8 and 12. ZYQL (100, 200 and 400 mg/kg) was administered via gavage daily for 14 days. The phagocytotic function of mononuclear phagocytic system was detected with carbon clearance methods, the levels of interleukin-6 (IL-6) and interferon-gamma (IFN-γ) in serum were detected with Enzyme linked immunosorbent assay (ELISA). Immune organs were weighed and organ indexes (organ weight/body weight) of thymus and spleen were calculated. Meanwhile, the activity of lysozyme (LSZ) in serum and the activity of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) in spleen tissue were measured.ResultsZYQL significantly upgrades the K value for clearance of carbon particles in normal mice treated with ZYQL (400 mg/kg) and immunosuppressed mice treated with ZYQL (100, 200 and 400 mg/kg) together with Cy (100 mg/kg) in vivo. The treatment of ZYQL (100, 200 and 400 mg/kg) effectively increased the activity of serum lysozyme as well as promoted the serum levels of IL-6 and IFN-γ in normal mice and immunosuppressed mice. Furthermore, ZYQL (100, 200 and 400 mg/kg) had an antioxidant effects in immune system by enhancing the antioxidant enzyme activity of SOD, CAT and GSH-Px in vivo. In addition, ZYQL (100, 200 and 400 mg/kg) effectively elevated the Cy-induced decreased organ index (thymus and spleen).ConclusionsThe present work shows that the dose-dependent administration of ZYQL is capable of influencing immune responses, which implying that its valuable functional health may be attributed partly to its protective effects for the immune function.

【 授权许可】

Unknown   
© Gao et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.