Effective cellular internalization of silica-coated CdSe quantum dots for high contrast cancer imaging and labelling applications

Cancer Nanotechnology

Research

Muthunayagam Vibin¹  Annie Abraham¹  Ramachandran Vinayakan²  Annie John³  Francis Boniface Fernandez³ 

[1] Department of Biochemistry, University of Kerala, Kariavattom campus, 695581, Thiruvananthapuram, Kerala, India;Photosciences and Photonics, National Institute for Interdisciplinary Science and Technology (CSIR), Thiruvananthapuram, Kerala, India;TEM Laboratory, Sree Chitra Tirunal Institute of Medical Sciences and Technology, Biomedical Technology Wing, Thiruvananthapuram, Kerala, India;

关键词: Stem cells;    Cellular imaging;    Fluorescence;    Internalization;    Quantum dots;    Ultrathin;   

DOI  :  10.1186/s12645-014-0001-y

 received in 2013-06-10, accepted in 2014-04-29,  发布年份 2014

来源: Springer

【 摘 要 】

The possibility of developing novel contrast imaging agents for cancer cellular labelling and fluorescence imaging applications were explored using silica-coated cadmium selenide (CdSe) quantum dots (QDs). The time dependent cellular internalization efficiency study was carried out using Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) and Confocal Laser Scanning Microscopy (cLSM) after exposing QDs to stem cells and cancer cells. The strong fluorescence from the cytoplasm confirmed that the QDs were efficiently internalized by the cells. The internalization maxima were observed at the fourth hour of incubation in both stem and cancer cells. Further, the in vitro fluorescence imaging as well as localization study of QDs were performed in various cells. Moreover, high contrast in vivo tumor imaging efficiency of silica-coated CdSe QDs was performed in ultrathin sections of tumor mice, and the results confirmed its effective role in cellular imaging and labelling in cancer and other diseases.

【 授权许可】

Unknown   
© Vibin et al.; licensee Springer 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.