Detection of IKKε by immunohistochemistry in primary breast cancer: association with EGFR expression and absence of lymph node metastasis

BMC Cancer

Research Article

Natalia Zamorano Cuervo¹  Nathalie Grandvaux²  Virginie Williams²  Marie-Pierre Sylvestre³  Andrée-Anne Grosset⁴  Yves St-Pierre⁵  Louis Gaboury⁶ 

[1] CRCHUM – Centre de recherche du Centre Hospitalier de l’Université de Montréal, 900 rue Saint-Denis, Qc H2X 0A9, Montréal, Canada;CRCHUM – Centre de recherche du Centre Hospitalier de l’Université de Montréal, 900 rue Saint-Denis, Qc H2X 0A9, Montréal, Canada;Department of Biochemistry and Molecular Medicine, Faculty of Medicine, Université de Montréal, Qc, Montréal, Canada;CRCHUM – Centre de recherche du Centre Hospitalier de l’Université de Montréal, 900 rue Saint-Denis, Qc H2X 0A9, Montréal, Canada;Department of Social and Preventive Medicine, Ecole de santé publique, Université de Montréal, Qc, Montréal, Canada;CRCHUM – Centre de recherche du Centre Hospitalier de l’Université de Montréal, 900 rue Saint-Denis, Qc H2X 0A9, Montréal, Canada;INRS-Institut Armand-Frappier, INRS, 531 Boul. des Prairies, Qc H7V 1B7, Laval, Canada;IRIC, Université de Montréal, 2900 Boul. Édouard-Montpetit, Montréal, H3T 1J4, Québec, Canada;INRS-Institut Armand-Frappier, INRS, 531 Boul. des Prairies, Qc H7V 1B7, Laval, Canada;IRIC, Université de Montréal, 2900 Boul. Édouard-Montpetit, Montréal, H3T 1J4, Québec, Canada;

关键词: IKKε;    Breast;    Cancer;    EGFR;    Metastasis;    Immunohistochemistry;    Biomarker;    Prognosis;   

DOI  :  10.1186/s12885-017-3321-6

 received in 2016-09-26, accepted in 2017-05-03,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundIKKε is an oncogenic kinase that was found amplified and overexpressed in a substantial percentage of human breast cancer cell lines and primary tumors using genomic and gene expression analyses. Molecular studies have provided the rational for a key implication of IKKε in breast cancer cells proliferation and invasiveness through the phosphorylation of several substrates.MethodsHere, we performed immunohistochemical detection of IKKε expression on tissue microarrays constituted of 154 characterized human breast cancer tumors. We further determined the association with multiple clinicopathological parameters and 5-years overall, disease-free and distant disease free survival.ResultsWe observed expression of IKKε in 60.4% of the breast cancer tumors. IKKε expression status showed no association with a panel of markers used for molecular classification of the tumors, including ER/PR/HER2 status, or with the molecular subtypes. However, IKKε expression was inversely associated with lymph node metastasis status (p = 0.0032). Additionally, we identified a novel association between IKKε and EGFR expression (p = 0.0011).ConclusionsThe unexpected observation of an inverse association between IKKε and lymph node metastasis advocates for larger scale immunohistochemical profiling of primary breast tumors to clarify the role of IKKε in metastasis. This study suggests that breast cancer tumors expressing EGFR and IKKε may be potential targets for drugs aiming at inhibiting IKKε activity or expression.

【 授权许可】

CC BY   
© The Author(s). 2017