Increased expression of carbonic anhydrase I in the synovium of patients with ankylosing spondylitis

BMC Musculoskeletal Disorders

Research Article

Xiaotian Chang¹  Yazhou Cui¹  Yan Zhao¹  Jinxiang Han¹  Shui Sun²  Xinfeng Yan³ 

[1] National Laboratory for Bio-Drugs of Ministry of Health, Provincial Laboratory for Modern Medicine and Technology of Shandong, Research Center for Medicinal Biotechnology of Shandong Academy of Medical Sciences, Jingshi road 18877, 250062, Jinan, Shandong, P.R. China;Orthopedic Surgery Center of Provincial Hospital of Shandong, Jinan, Shandong, P.R. China;Orthopedic Surgery Center of Shandong Qianfoshan Hospital, Jinan, Shandong, P.R. China;

关键词: Rheumatoid Arthritis;    Rheumatoid Arthritis Patient;    Ankylose Spondylitis;    Synovial Membrane;    Carbonic Anhydrase;   

DOI  :  10.1186/1471-2474-11-279

 received in 2010-05-06, accepted in 2010-12-08,  发布年份 2010

来源: Springer

【 摘 要 】

BackgroundOne of the most distinctive features of ankylosing spondylitis (AS) is new bone formation and bone resorption at sites of chronic inflammation. Previous studies have indicated that the hyperplasia and inflammation of synovial tissues are significantly related to the pathogenic process of AS. The present study used a proteomic approach to identify novel AS-specific proteins by simultaneously comparing the expression profiles of synovial membranes from patients with AS, rheumatoid arthritis (RA) and osteoarthritis (OA).MethodsSynovial tissues were collected from the hip joints of patients with AS and knee joints of patients with RA or OA (n = 10 for each disease) during joint replacement surgery. Proteins extracted from the synovial tissues were separated by 2-D electrophoresis (2-DE), and the proteins with significantly increased expression in the AS samples were subjected to MALDI-TOF/TOF-MS analysis. The results were verified using western blotting and immunohistochemistry. Levels of the candidate proteins in synovial fluids from knee joints (n = 40 for each disease) were measured using ELISA.ResultsThe proteomic approach revealed significantly increased expression of carbonic anhydrase I (CA1) in the synovial membrane of patients with AS as compared with the RA and OA tissue samples. Immunohistochemistry and western blotting analysis confirmed the findings described above. The ELISA detected a higher level of CA1 in synovial fluids from patients with AS than those with OA. The mean value of the CA1 level was also higher in AS patients as compared with RA patients. This study also detected increased expression of alpha-1-antitrypsin in the synovial tissues from AS patients, which is in agreement with other reports.ConclusionIn vitro experiments by other groups indicated that CA1 catalyzes the generation of HCO3- through the hydration of CO2, which then combines with Ca2+ to form a CaCO3 precipitate. Calcification is an essential step of bone formation. Substantial evidence indicates that carbonic anhydrase also stimulates bone resorption. Hence, overexpression of CA1 in the synovial tissues of AS patients may promote improper calcification and bone resorption in AS.

【 授权许可】

CC BY   
© Chang et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.