期刊论文详细信息
BMC Cancer
N-Acetylgalactosaminyltransferase-14 as a potential biomarker for breast cancer by immunohistochemistry
Research Article
Wenqian Song1  Hao Deng1  Xiaodan Guo1  Mingsheng Zhu1  Bo Zhang1  Yaojun Shan1  Chen Wu1  Jianfeng Ge1  Sisi Ma2  Weina Wang3  Yun Wang3 
[1] College of Life Sciences, Hebei University, 071002, Baoding, Hebei, P.R. China;College of Pharmaceutical Sciences, Hebei University, 071002, Baoding, Hebei, P.R. China;No. 3 Hospital, 071002, Baoding, Hebei, P.R. China;
关键词: Breast Cancer;    Invasive Ductal Carcinoma;    Oral Squamous Cell Carcinoma;    Mucinous Adenocarcinoma;    Breast Invasive Ductal Carcinoma;   
DOI  :  10.1186/1471-2407-10-123
 received in 2009-10-21, accepted in 2010-04-01,  发布年份 2010
来源: Springer
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【 摘 要 】

BackgroundThe post-translational modification of proteins, including glycosylation, differs between normal and tumor cells. The UDP-N-acetyl-D-galactosamine polypeptide N-acetylgalactosaminyltransferases (GalNAc-Tases) family of enzymes regulates the initial steps of mucin O-glycosylation and is responsible for the altered glycosylation state observed in cancer cells. Recently it was found that GalNAc-T14 mRNA is heterogeneously expressed in breast carcinomas compared to normal tissue, however the expression profile of GalNAc-T14 protein in breast carcinomas compared to normal tissue is still unknown. In this study, we assessed the expression profile of GalNAc-T14 protein in malignant and non-malignant breast tissues by immunohistochemistry to evaluate whether GalNAc-T14 might be a potential biomarker for breast cancer.MethodsIn formalin-fixed tissues, the expression level of GalNAc-T14 protein was evaluated by immunohistochemistry assay in breast tissues. Expression profiles were assessed in normal tissues, benign fibroadenomas and several types of carcinomas.ResultsOur results showed that GalNAc-T14 was heterogeneously expressed in breast carcinomas compared to non-malignant tissue. GalNAc-T14 expression was observed in 47/56 (83.9%) carcinoma samples, 7/48 (14.6%) non-malignant breast tissue samples. GalNAc-T14 expression level was associated with histological grade. For this enzyme a significant association with invasive ductal type, mucinous adenocarcinoma and ductal carcinoma in situ (DCIS) type was found.ConclusionOur results provide evidence that GalNAc-T14 may be a potential biomarker for breast cancer by immunohistochemistry. GalNAc-T14 expression level was associated with histological grade. GalNAc-T14 expression can provide new insights about breast cancer glycobiology.

【 授权许可】

CC BY   
© Wu et al; licensee BioMed Central Ltd. 2010

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