Genome sequence of Xanthomonas fuscans subsp. fuscansstrain 4834-R reveals that flagellar motility is not a general feature of xanthomonads

BMC Genomics

Research Article

Valérie Barbe¹  Stéphanie Fouteau¹  Stéphane Cociancich²  Isabelle Pieretti²  Philippe Rott²  Monique Royer²  Fabien Guérin³  Christian Vernière³  Olivier Pruvost³  Isabelle Robène-Soustrade³  Lionel Gagnevin³  Marie-Anne van Sluys⁴  Sébastien Carrère⁵  Endrick Guy⁵  Laurent D Noël⁵  Emmanuelle Lauber⁵  Matthieu Arlat⁶  Chrystelle Brin⁷  Marie-Agnès Jacques⁷  Laurana Serres-Giardi⁷  Martial Briand⁷  Armelle Darrasse⁷  Sophie Bonneau⁷  Karine Durand⁷  Tristan Boureau⁷  Arnaud Indiana⁷  Charles Manceau⁸  Stéphane Poussier⁹  Ralf Koebnik^1,10  Valérie Verdier^1,10  Boris Szurek^1,10  Alejandra Munoz^1,11  Mario L Arrieta-Ortiz^1,12 

[1] CEA, Genoscope, Centre National de Séquençage, F-91057, Evry Cedex, France;CIRAD, UMR BGPI, F-34398, Montpellier Cedex 5, France;CIRAD, UMR PVBMT, F-97410, Saint-Pierre, La Réunion, France;Université de la Réunion, UMR PVBMT, F-97715La Réunion, Saint-Denis, France;GaTE Lab Botanica IBUSP 277, 05508-900, São Paulo, SP, Brasil;INRA, LIPM UMR 441, F-31326, Castanet-Tolosan, France;CNRS, LIPM UMR 2594, F-31326, Castanet-Tolosan, France;INRA, LIPM UMR 441, F-31326, Castanet-Tolosan, France;CNRS, LIPM UMR 2594, F-31326, Castanet-Tolosan, France;Université de Toulouse, Université Paul Sabatier, UMR LIPM, F-31326, Castanet-Tolosan Cedex, France;INRA, UMR1345 Institut de Recherche en Horticulture et Semences, F-49071, Beaucouzé, France;AGROCAMPUS OUEST, UMR1345 Institut de Recherche en Horticulture et Semences, F-49045, Angers, France;Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV, PRES L’UNAM, F-49045, Angers, France;INRA, UMR1345 Institut de Recherche en Horticulture et Semences, F-49071, Beaucouzé, France;AGROCAMPUS OUEST, UMR1345 Institut de Recherche en Horticulture et Semences, F-49045, Angers, France;Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV, PRES L’UNAM, F-49045, Angers, France;ANSES, Laboratoire de Santé des végétaux, F-49044, Angers, France;INRA, UMR1345 Institut de Recherche en Horticulture et Semences, F-49071, Beaucouzé, France;AGROCAMPUS OUEST, UMR1345 Institut de Recherche en Horticulture et Semences, F-49045, Angers, France;Université d’Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV, PRES L’UNAM, F-49045, Angers, France;Université de la Réunion, UMR PVBMT, F-97715La Réunion, Saint-Denis, France;IRD, UMR RPB, F-34394, Montpellier Cedex 5, France;Universidad de Los Andes, Laboratorio de Micología y Fitopatología Uniandes, Bogotá, Colombia;Universidad de Los Andes, Laboratorio de Micología y Fitopatología Uniandes, Bogotá, Colombia;Department of Biology, Center for Genomics and Systems Biology, New York University, 10003, New York, NY, USA;

关键词: Seed-borne pathogen;    Secretion system;    Insertion sequence;    Bean;    Effector;    Chemotaxis;    Pseudogene;   

DOI  :  10.1186/1471-2164-14-761

 received in 2013-07-18, accepted in 2013-10-26,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundXanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome sequence of strain Xff 4834-R was determined and compared to other Xanthomonas genome sequences.ResultsComparative genomics analyses revealed core characteristics shared between Xff 4834-R and other xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters, secretion systems (from T1SS to T6SS) and multiple effectors. For instance a repertoire of 29 Type 3 Effectors (T3Es) with two Transcription Activator-Like Effectors was predicted. Mobile elements were associated with major modifications in the genome structure and gene content in comparison to other Xanthomonas genomes. Notably, a deletion of 33 kbp affects flagellum biosynthesis in Xff 4834-R. The presence of a complete flagellar cluster was assessed in a collection of more than 300 strains representing different species and pathovars of Xanthomonas. Five percent of the tested strains presented a deletion in the flagellar cluster and were non-motile. Moreover, half of the Xff strains isolated from the same epidemic than 4834-R was non-motile and this ratio was conserved in the strains colonizing the next bean seed generations.ConclusionsThis work describes the first genome of a Xanthomonas strain pathogenic on bean and reports the existence of non-motile xanthomonads belonging to different species and pathovars. Isolation of such Xff variants from a natural epidemic may suggest that flagellar motility is not a key function for in planta fitness.

【 授权许可】

Unknown   
© Darrasse et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.